(R,R)-PX20606
(R,R)-PX20606 is a FXR agonist with EC50s of 18 and 29 nM for FXR in FRET and M1H assay, respectively.
For research use only. We do not sell to patients.
- CAS No.: 1268244-88-7
- Formula: C29H22Cl3NO4
- Molecular Weight:554.85
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
EC50: 18 nM (FXR in FRET assay), 29 nM (FXR in M1H assay)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HEK293 | EC50 |
47 nM
Compound: 7b
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Agonist activity at C-terminal Gal4-tagged human FXR (187 to 472 residues) expressed in HEK-293 cells co-expressing pFRluc by mammalian one hybrid assay
Agonist activity at C-terminal Gal4-tagged human FXR (187 to 472 residues) expressed in HEK-293 cells co-expressing pFRluc by mammalian one hybrid assay
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[PMID: 27268696] |
Chemical Information
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CAS No. 1268244-88-7
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Appearance Solid
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Molecular Weight 554.85
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Formula C29H22Cl3NO4
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Color Light yellow to yellow
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SMILES
ClC1=C(C2=NOC(C3CC3)=C2COC4=CC=C([C@@H]5C[C@H]5C6=CC=C(C(O)=O)C=C6)C(Cl)=C4)C(Cl)=CC=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
Ethanol : ≥ 50 mg/mL (90.11 mM)
* "≥" means soluble, but saturation unknown.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.51 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (4.51 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
Purity & Documentation
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Data Sheet (275 KB)
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SDS (135 KB)
- English - EN (135 KB)
- Français - FR (135 KB)
- Deutsch - DE (135 KB)
- Norwegian - NO (135 KB)
- Español - ES (135 KB)
- Swedish - SV (135 KB)
- Italian - IT (135 KB)
- Korean - KR (135 KB)
- Portuguese - PT (135 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| Ethanol | 1 mM | 1.8023 mL | 9.0114 mL | 18.0229 mL | 45.0572 mL |
| 5 mM | 0.3605 mL | 1.8023 mL | 3.6046 mL | 9.0114 mL | |
| 10 mM | 0.1802 mL | 0.9011 mL | 1.8023 mL | 4.5057 mL | |
| 15 mM | 0.1202 mL | 0.6008 mL | 1.2015 mL | 3.0038 mL | |
| 20 mM | 0.0901 mL | 0.4506 mL | 0.9011 mL | 2.2529 mL | |
| 25 mM | 0.0721 mL | 0.3605 mL | 0.7209 mL | 1.8023 mL | |
| 30 mM | 0.0601 mL | 0.3004 mL | 0.6008 mL | 1.5019 mL | |
| 40 mM | 0.0451 mL | 0.2253 mL | 0.4506 mL | 1.1264 mL | |
| 50 mM | 0.0360 mL | 0.1802 mL | 0.3605 mL | 0.9011 mL | |
| 60 mM | 0.0300 mL | 0.1502 mL | 0.3004 mL | 0.7510 mL | |
| 80 mM | 0.0225 mL | 0.1126 mL | 0.2253 mL | 0.5632 mL |
(R,R)-PX20606 Related Classifications
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