1. Metabolic Enzyme/Protease
  2. Endogenous Metabolite
  3. N-Decanoyl p-Nitroaniline

N-Decanoyl p-Nitroaniline  (Synonyms: DepNA)

Art. -Nr.: HY-120971 Reinheit: 99.9%
Handling Instructions Technical Support

N-Decanoyl p-nitroaniline (DepNA) is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity.1 FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its endogenous substrate anandamide (AEA). It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. Exposure of DepNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε=13,500 at 410 nm). This allows the fast and convenient measurement of FAAH activity using a 96 well plate spectrophotometer.

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N-Decanoyl p-Nitroaniline

N-Decanoyl p-Nitroaniline Chemische Struktur

CAS. Nr. : 72298-63-6

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Beschreibung

N-Decanoyl p-nitroaniline (DepNA) is one of several nitroaniline fatty acid amides which can be used to measure fatty acid amide hydrolase (FAAH) activity.1 FAAH is a relatively unselective enzyme in that it accepts a variety of amide head groups other than the ethanolamine of its endogenous substrate anandamide (AEA). It also will hydrolyze fatty acid amides with fewer carbons and fewer double bonds than arachidonate. Exposure of DepNA to FAAH activity results in the release of the yellow colorimetric dye p-nitroaniline (ε=13,500 at 410 nm). This allows the fast and convenient measurement of FAAH activity using a 96 well plate spectrophotometer.

Molekulargewicht

292.37

Formel

C16H24N2O3

CAS. Nr.
Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCCCC(NC1=CC=C([N+]([O-])=O)C=C1)=O

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
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Die Formel zur Berechnung von Molaritäten

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
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Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)

Diese Gleichung wird häufig abgekürzt als: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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N-Decanoyl p-Nitroaniline
Art. -Nr.:
HY-120971
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