N-trans-Feruloyl-3-methyldopamine
Based on 1 Customer Validation
N-trans-Feruloyl-3-methyldopamine (compound 4) is an anticancer compound isolated from Alternanthera philoxeroides. N-trans-Feruloyl-3-methyldopamine is cytotoxic to HeLa cells, with an inhibition rate of 72.2% at a concentration of 30 μg/mL.
For research use only. We do not sell to patients.
- Purity: 99.02%
- CAS No.: 78510-19-7
- Formula: C19H21NO5
- Molecular Weight:343.37
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| BV-2 | IC50 |
>50 μM
Compound: 29
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Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 20 hrs by Griess assay
Antineuroinflammatory activity against mouse BV2 cells assessed as inhibition of LPS-induced NO production incubated for 20 hrs by Griess assay
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[PMID: 33822610] |
| HepG2 | IC50 |
>20 μg/mL
Compound: trans-N-feruloyl-3-o-methyldopamine
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Growth inhibition of HepG2 cells
Growth inhibition of HepG2 cells
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[PMID: 17547458] |
| RAW264.7 | IC50 |
>50 μM
Compound: 26
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Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay
Antiinflammatory activity in mouse RAW264.7 assessed as inhibition of LPS-induced NO production preincubated for 30 mins followed by LPS stimulation measured after 24 hrs by Griess reagent based assay
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[PMID: 33939429] |
| WI-38 | IC50 |
>20 μg/mL
Compound: trans-N-feruloyl-3-o-methyldopamine
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Growth inhibition of WI38 cells
Growth inhibition of WI38 cells
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[PMID: 17547458] |
| WI-38 VA13 | IC50 |
>20 μg/mL
Compound: trans-N-feruloyl-3-o-methyldopamine
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Growth inhibition of VA13 cells
Growth inhibition of VA13 cells
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[PMID: 17547458] |
Chemical Information
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CAS No. 78510-19-7
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Appearance Solid
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Molecular Weight 343.37
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Formula C19H21NO5
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Color Light yellow to yellow
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SMILES
COC1=C(O)C=CC(/C=C/C(NCCC2=CC(OC)=C(O)C=C2)=O)=C1
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (291.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (7.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (7.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9123 mL | 14.5616 mL | 29.1231 mL | 72.8078 mL |
| 5 mM | 0.5825 mL | 2.9123 mL | 5.8246 mL | 14.5616 mL | |
| 10 mM | 0.2912 mL | 1.4562 mL | 2.9123 mL | 7.2808 mL | |
| 15 mM | 0.1942 mL | 0.9708 mL | 1.9415 mL | 4.8539 mL | |
| 20 mM | 0.1456 mL | 0.7281 mL | 1.4562 mL | 3.6404 mL | |
| 25 mM | 0.1165 mL | 0.5825 mL | 1.1649 mL | 2.9123 mL | |
| 30 mM | 0.0971 mL | 0.4854 mL | 0.9708 mL | 2.4269 mL | |
| 40 mM | 0.0728 mL | 0.3640 mL | 0.7281 mL | 1.8202 mL | |
| 50 mM | 0.0582 mL | 0.2912 mL | 0.5825 mL | 1.4562 mL | |
| 60 mM | 0.0485 mL | 0.2427 mL | 0.4854 mL | 1.2135 mL | |
| 80 mM | 0.0364 mL | 0.1820 mL | 0.3640 mL | 0.9101 mL | |
| 100 mM | 0.0291 mL | 0.1456 mL | 0.2912 mL | 0.7281 mL |
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