2. Apoptosis
  3. Apoptosis Caspase TNF Receptor
  4. OH14

OH14 is a cellular FLICE-like inhibitory protein (cFLIP) inhibitor and TNF-related apoptosis-inducing ligand (TRAIL) sensitizer. OH14 selectively binds to the DED1 pocket of cFLIP, disrupting its recruitment to the TRAIL-death inducing signalling complex without affecting procaspase-8 recruitment to FADD, allowing procaspase-8 activation. OH14 promotes TRAIL-mediated apoptosis and impairs cell viability in breast cancer systems when combined with TRAIL. OH14 can be used for the research of breast cancer.

For research use only. We do not sell to patients.

OH14

OH14 Chemical Structure

CAS No. : 1018153-87-1

Size Stock
50 mg   Get quote  
100 mg   Get quote  
250 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

OH14 Related Antibodies

Top Publications Citing Use of Products

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

View All TNF Receptor Isoform Specific Products:

View All Isoforms
TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF10B/DR5/CD262 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

OH14 is a cellular FLICE-like inhibitory protein (cFLIP) inhibitor and TNF-related apoptosis-inducing ligand (TRAIL) sensitizer. OH14 selectively binds to the DED1 pocket of cFLIP, disrupting its recruitment to the TRAIL-death inducing signalling complex without affecting procaspase-8 recruitment to FADD, allowing procaspase-8 activation. OH14 promotes TRAIL-mediated apoptosis and impairs cell viability in breast cancer systems when combined with TRAIL. OH14 can be used for the research of breast cancer[1].

In Vitro

OH14 (75-100 μM; 1 h) sensitizes MCF-7 breast cancer cells and HeLa cells to TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis in a dose-dependent manner, as measured by increased cleaved caspase 3/7 activity and Annexin V staining, respectively[1].
OH14 (100 μM; 1 h) requires the R38 residue in cFLIP to sensitize HeLa cells to TRAIL-induced apoptosis, while overexpression of wild-type, H7A, or K18I/R45A mutant cFLIP does not impair this sensitizing activity[1].
OH14 (100 μM; 1 h) significantly sensitizes MCF-7, BT474, and MDA-MB-231 breast cancer cell lines to TRAIL-induced cell death, with variable effects across other breast cancer subtypes[1].
OH14 (100 μM; 1 h) disrupts TRAIL-mediated recruitment of cFLIP to FADD in HeLa cells, as measured by loss of FRET signal between cFLIP-YFP and FADD-CFP[1].
OH14 (100 μM; 1 h) specifically disrupts TRAIL-mediated recruitment of cFLIP to the FADD complex in MCF-7 cells, while allowing procaspase-8 cleavage and activation[1].
OH14 (10 μM; 10 days) sensitizes MCF-7 breast cancer cells to TRAIL-induced reduction in colony formation, without affecting colony growth when administered alone[1].
OH14 (10 μM; 24 h) is non-toxic to HEK293 non-tumorigenic human embryonic kidney cells, either alone or in combination with TRAIL[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

OH14 Related Antibodies

Apoptosis Analysis[1]

Cell Line: MCF-7 breast cancer cells, HeLa cells
Concentration: 75 μM, 100 μM
Incubation Time: 1 h pre-incubation, followed by 24 h TRAIL treatment
Result: Observed a dose-dependent sensitization effect.
Significantly increased cleaved caspase 3/7 activity in MCF-7 cells.
Significantly increased Annexin V staining in HeLa cells.

Cell Viability Assay[1]

Cell Line: Panel of breast cancer cell lines: MCF-7, BT474, MDA-MB-231, HCC1954, SUM149
Concentration: 100 μM
Incubation Time: 1 h pre-incubation, followed by 18 h TRAIL treatment
Result: Significantly sensitized MCF-7, BT474, and MDA-MB-231 breast cancer cell lines to TRAIL.
Observed similar but statistically insignificant trends in HCC1954 and SUM149 cells.

Western Blot Analysis[1]

Cell Line: MCF-7 breast cancer cells
Concentration: 100 μM
Incubation Time: 1 h pre-incubation, followed by 2 h TRAIL treatment
Result: Disrupted the TRAIL-induced interaction between FADD and both short and long forms of cFLIP.
Did not inhibit the interaction between FADD and procaspase-8.
Increased the ratio of cleaved cFLIP (43 kDa) to noncleaved cFLIP (55 kDa).
Induced cleavage of procaspase-8 to its active 43 kDa form when combined with TRAIL.
Molecular Weight

360.21

Formula

C14H11Cl2NO4S
CAS No.
SMILES

CC1=C(C=C(C(S(=O)(NC2=CC=CC=C2C(O)=O)=O)=C1)Cl)Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

OH14 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

OH141018153-87-1OH 14OH-14ApoptosisCaspaseTNF ReceptorTumor Necrosis Factor ReceptorTNFRHeLa cellsDED1 pocketcFLIPbreast cancerMCF-7 breast cancer cellsFADDTRAILprocaspase-8BT474 breast cancer cell linesMDA-MB-231 breast cancer cell linesInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
OH14
Cat. No.:
HY-182307
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.