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Olomoucine II

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Olomoucine II is a potent CDK inhibitor with IC₅₀ values of 0.06, 0.1, 0.45, 7.6, 19.8 µM for CDK9/cyclin T, CDK2/cyclin E, CDK7/cyclin H, CDK1/cyclin B, CDK4/cyclin D1, respectively. Olomoucine II shows antiproliferative activity.

For research use only. We do not sell to patients.

CAS No.: 500735-47-7
Formula: C₁₉H₂₆N₆O₂
Molecular Weight:370.45

Storage:

Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month

Olomoucine II Chemical Structure

CAS No.: 500735-47-7

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

CDK9/cycT

0.06 μM (IC₅₀)

CDK2/cyclinE

0.1 μM (IC₅₀)

CDK7/cyclin H

0.45 μM (IC₅₀)

Cdk1/cyclin B

7.6 μM (IC₅₀)

CDK4/cyclinD1

19.8 μM (IC₅₀)

All CDK Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
BJ	EC₅₀	5.4 μM Compound: olomoucine II	Cytotoxicity against human BJ cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human BJ cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
CCRF-CEM	EC₅₀	8.1 μM Compound: olomoucine II	Cytotoxicity against human CEM cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human CEM cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
CCRF-CEM	IC₅₀	3 μM Compound: Olomoucine II	In vitro cytotoxic effect on CEM cancer cell line In vitro cytotoxic effect on CEM cancer cell line	[PMID: 12392733]
CCRF-CEM	IC₅₀	7.4 μM Compound: Olomoucine II	Cytotoxicity against human CEM cells assessed as reduction in number of viable cells after 72 hrs by fluorescence assay Cytotoxicity against human CEM cells assessed as reduction in number of viable cells after 72 hrs by fluorescence assay	[PMID: 22770608]
G-361	IC₅₀	6.3 μM Compound: Olomoucine II	In vitro cytotoxic effect on G361 cancer cell line In vitro cytotoxic effect on G361 cancer cell line	[PMID: 12392733]
G-361	IC₅₀	9.2 μM Compound: Olomoucine II	Cytotoxicity against human G361 cells assessed as reduction in number of viable cells after 72 hrs by fluorescence assay Cytotoxicity against human G361 cells assessed as reduction in number of viable cells after 72 hrs by fluorescence assay	[PMID: 22770608]
HCT-116	IC₅₀	4.1 μM Compound: Olomoucine II	Cytotoxicity against human HCT116 cells assessed as reduction in number of viable cells after 72 hrs by fluorescence assay Cytotoxicity against human HCT116 cells assessed as reduction in number of viable cells after 72 hrs by fluorescence assay	[PMID: 22770608]
HeLa	EC₅₀	9.9 μM Compound: olomoucine II	Cytotoxicity against human HeLa cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human HeLa cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
HOS	IC₅₀	10.6 μM Compound: Olomoucine II	Cytotoxicity against human HOS cells assessed as reduction in number of viable cells after 72 hrs by fluorescence assay Cytotoxicity against human HOS cells assessed as reduction in number of viable cells after 72 hrs by fluorescence assay	[PMID: 22770608]
HOS	IC₅₀	6.3 μM Compound: Olomoucine II	In vitro cytotoxic effect on HOS cancer cell line In vitro cytotoxic effect on HOS cancer cell line	[PMID: 12392733]
K562	IC₅₀	11.1 μM Compound: Olomoucine II	In vitro cytotoxic effect on K562 cancer cell line In vitro cytotoxic effect on K562 cancer cell line	[PMID: 12392733]
K562	IC₅₀	23 μM Compound: Olomoucine II	Cytotoxicity against human K562 cells assessed as reduction in number of viable cells after 72 hrs by fluorescence assay Cytotoxicity against human K562 cells assessed as reduction in number of viable cells after 72 hrs by fluorescence assay	[PMID: 22770608]
MCF7	EC₅₀	4.6 μM Compound: olomoucine II	Cytotoxicity against human MCF7 cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human MCF7 cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
MCF7	IC₅₀	5.3 μM Compound: Olomoucine II	In vitro cytotoxic effect on MCF-7 cancer cell line In vitro cytotoxic effect on MCF-7 cancer cell line	[PMID: 12392733]
MCF7	IC₅₀	9.1 μM Compound: Olomoucine II	Cytotoxicity against human MCF7 cells assessed as reduction in number of viable cells after 72 hrs by fluorescence assay Cytotoxicity against human MCF7 cells assessed as reduction in number of viable cells after 72 hrs by fluorescence assay	[PMID: 22770608]
RPMI-8226	EC₅₀	6 μM Compound: olomoucine II	Cytotoxicity against human RPMI8226 cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human RPMI8226 cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]
Sf9	IC₅₀	50 nM Compound: Olomoucine II	Inhibition of CDK2/Cyclin E (unknown origin) expressed in Sf9 cells using histone H1 as substrate Inhibition of CDK2/Cyclin E (unknown origin) expressed in Sf9 cells using histone H1 as substrate	[PMID: 22770608]
U-266	EC₅₀	7.2 μM Compound: olomoucine II	Cytotoxicity against human U266 cells assessed as viable cells after 72 hrs by calcein AM assay Cytotoxicity against human U266 cells assessed as viable cells after 72 hrs by calcein AM assay	[PMID: 20192247]

In Vitro

Olomoucine II (72 h) shows antiproliferative activity with IC₅₀s of 9.3, 9.2, 10.5, 13.6, 5.0, 10.8, 5.3, 2.7, 16.3 µM for HOS, T98G, HBL100, BT474, MCF-7, HT-29, CCRF-CEM, BV173, HL60 cells, respectively^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

HOS, T98G, HBL100, BT474, MCF-7, HT-29, CCRF-CEM, BV173, HL60 cells
Concentration:
Incubation Time:

72 h
Result:

Showed antiproliferative activity with IC₅₀s of 9.3, 9.2, 10.5, 13.6, 5.0, 10.8, 5.3, 2.7, 16.3 µM for HOS, T98G, HBL100, BT474, MCF-7, HT-29, CCRF-CEM, BV173, HL60 cells, respectively.

Chemical Information

CAS No. 500735-47-7
Appearance Solid
Molecular Weight 370.45
Formula C₁₉H₂₆N₆O₂
Color White to off-white
SMILES

OC1=CC=CC=C1CNC2=C3N=CN(C(C)C)C3=NC(N[C@@H](CO)CC)=N2
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
In solvent	-80°C	6 months
	-20°C	1 month

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (274 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Krystof V, et al. Antiproliferative activity of olomoucine II, a novel 2,6,9-trisubstituted purine cyclin-dependent kinase inhibitor. Cell Mol Life Sci. 2005 Aug;62(15):1763-71. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

100 mg

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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Olomoucine II

High-Throughput Bioactive Compound Library

All CDK Isoforms