Poly(G)  (Synonyms: Polyguanylic acid)

Poly (G) (Polyguanylic acid) is a high-molecular-weight synthetic ribopolynucleotide. In the presence of Na⁺ or K⁺ ions, Poly (G) undergoes time-dependent structural modification, gradually self-assembling from a single strand into large G-quadruplex aggregates. Poly (G) undergoes cooperative secondary structure dissociation under alkaline conditions, but its secondary structure can withstand high temperatures up to 100°C and is resistant to snake venom diesterase at low Na⁺ concentrations. Poly (G) forms stable 1:1 complexes with polycytidylic acid or poly 5-bromocytidylic acid, but does not bind to polyinosinic acid or polyadenylic acid. Poly (G) can be applied to studies on the interactions between drugs and G-rich DNA fragments in electrochemical biosensors^[1][2].

Poly (G) (5 μg/mL; 3 min) undergoes time-dependent structural modification on the HOPG surface in the presence of Na⁺ or K⁺ ions, transforming from a single-stranded structure at 0 h to short GQ domains at 24 h, then to large, low-adsorptivity GQ assemblies at 21 days, which ultimately leads to a time-dependent decrease in HOPG surface coverage^[1].
Poly (G) (100 μg/mL; 0-21 d) exhibits time-dependent redox behavior on the surface of glassy carbon electrode (GCE) in the presence of Na⁺ or K⁺ ions: the single-stranded guanine (G) oxidation peak observed at 0 h converts to a G-quadruplex (GQ) oxidation peak that reaches a current maximum on day 10 and stabilizes after approximately 17 days, with the G oxidation peak completely disappearing by day 21^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

(C₁₀H₁₄N₅O₈P)x

25191-14-4

O=C1C(N=CN2[C@H]3[C@H](O)[C@H](O)[C@@H](COP(O)(O)=O)O3)=C2N=C(N)N1.[n]

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Chiorcea-Paquim A M, et al. Time-dependent polyguanylic acid structural modifications[J]. Electrochemistry communications, 2014, 45: 71-74.

  [2]. Pochon F, Michelson A M. Polynucleotides. VI. Interaction between polyguanylic acid and polycytidylic acid[J]. Proceedings of the National Academy of Sciences, 1965, 53(6): 1425-1430.