Bioactive constituents of the roots of Cynanchum atratum

  • J Nat Prod. 2001 May;64(5):608-11. doi: 10.1021/np000428b.
S H Day  1 ,  J P Wang ,  S J Won ,  C N Lin
Affiliations
  • 1. School of Pharmacy, Kaohsiung Medical University, Kaohsiung, Taiwan 807, Republic of China.
PMID: 11374953 DOI: 10.1021/np000428b
Abstract

A novel biphenylneolignan, 2,6,2',6'-tetramethoxy-4,4'-bis(2,3-epoxy-1-hydroxypropyl)biphenyl (1), and two new glycosides named atratoglaucosides A (2) and B (3), were isolated from the roots of Cynanchum atratum, and their structures were determined on the basis of chemical and spectroscopic evidence. The aglycons of 2 and 3 were identified as glaucogenin C and 7-desoxyneocynapanogenin A, a new disecopregnane. A known compound, glaucogenin C 3-O-beta-D-cymaropyranosyl-(1-->4)-alpha-L-diginopyranosyl-(1-->4)-beta-D-thevetopyranoside (4), isolated from the same source, showed a significant cytotoxic effect against 212 cells. This substance also gave a significant inhibitory effect on TNF-alpha (tumor necrosis factor-alpha) formation from the RAW 264.7 mouse macrophage-like cell line stimulated with LPS (lipopolysaccharide) and on the N9 microglial cell line stimulated with LPS/IFN-gamma (interferon-gamma).

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