Glycerolipids as selective thrombin inhibitors from the fungus Stereum hirsutum

  • Drug Dev Ind Pharm. 2006 Jun;32(5):635-43. doi: 10.1080/03639040500530026.
Bojan Doljak  1 ,  Francesca Cateni ,  Marko Anderluh ,  Giuseppe Procida ,  Jelena Zilic ,  Marina Zacchigna
Affiliations
  • 1. Department of Pharmaceutical Biology, Faculty of Pharmacy, University of Ljubljana, Askerceva 7, 1000 Ljubljana, Slovenia.
PMID: 16720417 DOI: 10.1080/03639040500530026
Abstract

A dry extract of Stereum hirsutum exhibited a significant inhibitory activity on Thrombin (34%). A bioassay oriented fractionation of the extract of Stereum hirsutum has led to the isolation of two active fractions. On the basis of spectroscopic data, chemical reactions, and GC-MS analysis, complex mixtures of diacylglycerophospholipids (DAGPs) and diacylglycerols (DGs) have been isolated and identified. Docking studies suggest that some isolated compounds could bind to the Thrombin active site in a similar manner as previously reported phosphonate Thrombin inhibitors.

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