Determination of the theophylline solubilizer salicylamide-O-acetic acid in serum and urine using high-performance liquid chromatography

  • J Pharm Biomed Anal. 1985;3(5):469-75. doi: 10.1016/0731-7085(85)80062-5.
H U Schulz  1 ,  E Kraas
Affiliations
  • 1. Department of Angiology and Geriatrics, Medical University Lübeck, Ratzeburger Allee 160, D-2400 Lübeck, Federal Republic of Germany.
PMID: 16867660 DOI: 10.1016/0731-7085(85)80062-5
Abstract

A high-performance liquid chromatographic method for the determination of the theophylline solubilizer salicylamide-O-acetic acid has been developed in the range 0.5 to 10 microg/ml for human serum and 5 to 400 microg/ml for urine. Reversed-phase ion-pair chromatography was employed with tetrabutylammonium hydrogen sulphate as counterion and 2-nitrophenylacetic acid as internal standard. Preliminary pharmacokinetic single-dose studies show that the sensitivity and the selectivity of the assay are adequate to measure lower concentrations in the late beta-phase.

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