Large-scale high-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

  • Nat Protoc. 2007;2(1):38-41. doi: 10.1038/nprot.2007.15.
R Daniel Gietz  1 ,  Robert H Schiestl
Affiliations
  • 1. Department of Biochemistry and Medical Genetics, University of Manitoba, T250-770 Bannatyne Ave., Winnipeg, Manitoba R3E 0W3, Canada.
PMID: 17401336 DOI: 10.1038/nprot.2007.15
Abstract

Here, we describe a Library screen transformation protocol using the lithium acetate/single-stranded carrier DNA/PEG method of transformation for Saccharomyces cerevisiae. This method is suitable for screening complex plasmid libraries such as those used for yeast two-hybrid analysis. This procedure takes up to 2.5 h to complete once the yeast culture has been grown.

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