Steroidal saponins from Yucca gloriosa L. rhizomes: LC-MS profiling, isolation and quantitative determination

  • Phytochemistry. 2011 Jan;72(1):126-35. doi: 10.1016/j.phytochem.2010.10.013.
Alexandre Skhirtladze  1 ,  Angela Perrone ,  Paola Montoro ,  Mariam Benidze ,  Ether Kemertelidze ,  Cosimo Pizza ,  Sonia Piacente
Affiliations
  • 1. Iovel Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia.
PMID: 21094503 DOI: 10.1016/j.phytochem.2010.10.013
Abstract

The occurrence of steroidal saponins in the rhizomes of Yucca gloriosa has been detected by LC-MS. On the basis of the LC-MS analysis, five steroidal glycosides, including three spirostane, one furostane and one cholestane glycosides, along with seven known compounds have been isolated and characterized by ESI-MS and by the extensive use of 1D- and 2D-NMR experiments. Quantitative analysis of the steroidal glycosides in Y. gloriosa rhizomes was performed by an LC-MS method validated according to European Medicines Agency (EMEA) guidelines. The dried BuOH extract obtained from rhizomes contains more than 25% w/w of glycosides, thus Y. gloriosa rhizomes can be considered a rich source of steroidal glycosides.

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