Chemically Controlled Epigenome Editing through an Inducible dCas9 System

  • J Am Chem Soc. 2017 Aug 23;139(33):11337-11340. doi: 10.1021/jacs.7b06555.
Tingjun Chen  1 ,  Dan Gao  1 ,  Roushu Zhang  1 ,  Guihua Zeng  1 ,  Hao Yan  1 ,  Eunju Lim  1 ,  Fu-Sen Liang  1
Affiliations
  • 1. Department of Chemistry and Chemical Biology, University of New Mexico , 300 Terrace Street NE, Albuquerque, New Mexico 87131, United States.
PMID: 28787145 DOI: 10.1021/jacs.7b06555
Abstract

Although histone modifications are associated with gene activities, studies of their causal relationships have been difficult. For this purpose, we developed an inducible system integrating dCas9-based targeting and chemically induced proximity technologies to allow small molecule induced recruitment of P300 acetyltransferase and the acetylation of H3K27 at precise gene loci in cells. Employing the new technique, we elucidated the temporal order of histone acetylation and gene activation, as well as the stability of the installed histone modification.