Bioactive Diterpenoids from the Stems of Euphorbia royleana

  • J Nat Prod. 2019 Feb 22;82(2):183-193. doi: 10.1021/acs.jnatprod.8b00493.
Peixia Wang  1 ,  Chunfeng Xie  1 ,  Lijun An  1 ,  Xueyuan Yang  1 ,  Yaru Xi  1 ,  Shuo Yuan  1 ,  Chenyue Zhang  1 ,  Muhetaer Tuerhong  2 ,  Da-Qing Jin  3 ,  Dongho Lee  4 ,  Jie Zhang  5 ,  Yasushi Ohizumi  6 ,  Jing Xu  1 ,  Yuanqiang Guo  1
Affiliations
  • 1. State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research , Nankai University , Tianjin 300350 , People's Republic of China.
  • 2. College of Chemistry and Environmental Sciences, Laboratory of Xinjiang Native Medicinal and Edible Plant Resources Chemistry , Kashgar University , Kashgar 844000 , People's Republic of China.
  • 3. School of Medicine , Nankai University , Tianjin 300071 , People's Republic of China.
  • 4. Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology , Korea University , Seoul 02841 , Republic of Korea.
  • 5. Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering , Shihezi University , Shihezi 832003 , People's Republic of China.
  • 6. Kansei Fukushi Research Institute , Tohoku Fukushi University , Sendai 989-3201 , Japan.
PMID: 30730729 DOI: 10.1021/acs.jnatprod.8b00493
Abstract

Two ingenane- (1 and 2), two ent-atisane- (3 and 4), two ent-kaurane- (5 and 6), two ent-abietane- (7 and 8), and one ent-isopimarane-type (9) diterpenoid and 12 known analogues have been isolated from the methanolic extract of the stems of Euphorbia royleana. Their structures, including absolute configurations, were determined by extensive spectroscopic methods and ECD data analysis. The nitric oxide inhibitory activities of those Diterpenoids were examined biologically in lipopolysaccharide-stimulated BV-2 cells, with compounds 1, 2, 5-7, 10, and 12 having IC50 values lower than 40 μM. Molecular docking was used to investigated the possible mechanism of compounds 1, 2, 5-7, 10, and 12.