MnO2 nanoflowers based colorimetric and fluorescent dual-mode aptasensor for sensitive detection of aflatoxin B1 in milk

  • Anal Chim Acta. 2023 Oct 23:1279:341844. doi: 10.1016/j.aca.2023.341844.
Shuofeng Li  1 ,  Fangfang Wang  2 ,  Bin Zhao  3 ,  Chun Wang  3 ,  Zhi Wang  1 ,  Qiuhua Wu  4
Affiliations
  • 1. College of Food Science and Technology, Hebei Agricultural University, Baoding, 071001, China; College of Science, Hebei Agricultural University, Baoding, 071001, China.
  • 2. Hebei Bioinformatic Utilization and Technological Innovation Center for Agricultural Microbes, College of Life Sciences, Hebei Agricultural University, Baoding, 071001, China. Electronic address: [email protected].
  • 3. College of Science, Hebei Agricultural University, Baoding, 071001, China.
  • 4. College of Food Science and Technology, Hebei Agricultural University, Baoding, 071001, China; College of Science, Hebei Agricultural University, Baoding, 071001, China. Electronic address: [email protected].
PMID: 37827626 DOI: 10.1016/j.aca.2023.341844
Abstract

Aflatoxin B1 (AFB1) with tremendous toxic effects has caused a serious threat to food security. Accurate quantification of AFB1 in food can effectively prevent the risk of human intake of AFB1. Herein, a colorimetric and fluorescent dual-mode aptasensor for accurate and sensitive detection of AFB1 has been developed based on MnO2 nanoflowers (MnO2NFs) for the first time. MnO2NFs could catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) into blue oxidation product (TMBox) by H2O2, which would be used for visible detection of AFB1. Simultaneously, MnO2NFs can be served as a signal amplifier and reduced by ascorbic acid to generate lots of Mn2+ which would quench the fluorescence of calcein for fluorescent detection of AFB1. Both colorimetric and fluorescent methods have been successfully applied for determination of AFB1 in milk samples with satisfactory results. The proposed dual-mode detection method with high detection sensitivity and accuracy showed great promise for monitoring AFB1 in food.

Keywords
Aflatoxin B1; Aptasensor; Dual-mode detection; MnO(2) nanoflowers.
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