Epitranscriptomic analysis reveals features of NAD-capped RNAs upon supplementation of nicotinamide mononucleotide in human

  • Exp Cell Res. 2025 Oct 8;453(1):114780. doi: 10.1016/j.yexcr.2025.114780.
Shuwen Ge  1 ,  Yandong Liu  2 ,  Dean Li  1 ,  Guojing Han  2 ,  Kongyan Niu  1 ,  Hujun Ju  3 ,  Hao Zhou  4 ,  Rui Liu  5 ,  Zhengjiang Zhu  6 ,  Nan Liu  7 ,  Lefeng Qu  8
Affiliations
  • 1. Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 100 Hai Ke Rd., Pudong, Shanghai, 201210, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
  • 2. Department of Vascular and Endovascular Surgery, Chang Zheng Hospital, Naval Medical University, Shanghai, 200003, China.
  • 3. Redpont Biotechnology, Hongtaiyang Life Science Industrial Park, Dangtu Economic Development Zone, Ma'anshan, Anhui Province, 243000, China.
  • 4. Haven Biotechnology, 1 Jing Ba Rd, Economic Development Zone, Ganzhou, Jiangxi Province, 342499, China.
  • 5. Singlera Genomics, 500 Fu Rong Hua Rd., Pudong, Shanghai, 201204, China.
  • 6. Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 100 Hai Ke Rd., Pudong, Shanghai, 201210, China; Shanghai Key Laboratory of Aging Studies, 100 Hai Ke Rd., Pudong, Shanghai, 201210, China.
  • 7. Interdisciplinary Research Center on Biology and Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 100 Hai Ke Rd., Pudong, Shanghai, 201210, China; Shanghai Key Laboratory of Aging Studies, 100 Hai Ke Rd., Pudong, Shanghai, 201210, China. Electronic address: [email protected].
  • 8. Department of Vascular and Endovascular Surgery, Chang Zheng Hospital, Naval Medical University, Shanghai, 200003, China. Electronic address: [email protected].
PMID: 41072840 DOI: 10.1016/j.yexcr.2025.114780
Abstract

Nicotinamide mononucleotide (NMN), a precursory metabolite of NAD, has been demonstrated to boost cellular NAD level that is coupled with various age-related beneficial effects in animal models. NAD-capped RNA (NAD-RNA) represents a critical but poorly studied modification at the epitranscriptomic level. Here we examine the impact of NMN supplementation on NAD-RNA in human peripheral blood mononuclear cells (PBMCs). We demonstrated that NMN supplementation increases NAD turnover coupled with a reduction in NAD-capped RNAs in both human and dog, revealing blood-derived NAD-RNAs as potential biomarkers sensitized to NMN exposure.

Keywords
Epitranscriptome; NAD-Capped RNAs; NMN; PBMC.
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