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  4. Pyruvate Oxidase, Microorganisms

Pyruvate Oxidase, Microorganisms (PoxB) is a peripheral membrane enzyme, isolated from microorganisms. Pyruvate oxidase catalyzes the oxidative decarboxylation of pyruvate to acetyl phosphate, carbon dioxide and water. Pyruvate oxidase is an important enzyme in bacterial metabolism and is often used in biochemical research.

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Pyruvate Oxidase, Microorganisms

Pyruvate Oxidase, Microorganisms Chemical Structure

CAS No. : 9001-96-1

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Pyruvate Oxidase, Microorganisms Related Antibodies

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Description

Pyruvate Oxidase, Microorganisms (PoxB) is a peripheral membrane enzyme, isolated from microorganisms. Pyruvate oxidase catalyzes the oxidative decarboxylation of pyruvate to acetyl phosphate, carbon dioxide and water. Pyruvate oxidase is an important enzyme in bacterial metabolism and is often used in biochemical research[1][2][3][4][5].

In Vitro

Pyruvate Oxidase contributes to stationary-phase acetate accumulation in Escherichia coli K-12 strain JRG3456[2].
Pyruvate Oxidase (50 mM phosphate buffer; 10 min) has an initial activity optimum at 38°C, but is unstable at temperatures above 30°C, with activity declining after 10 min of incubation at temperatures exceeding 30°C[4].
Pyruvate Oxidase (0.1-150 mM) exhibits highest activity with pyruvate, reacts with methylglyoxal and acetaldehyde[4].
Pyruvate Oxidase (1-10 mM) is only slightly inhibited by 10 mM EDTA (11%) and 10 mM KCN (25%), and is unaffected by 10 mM NaN3, 1 mM iodoacetamide, or 1 mM p-hydroxymercuribenzoate[4].
Pyruvate oxidase requires TPP, FAD, and a divalent cation (e.g., Mn2+) to catalyze the four-step oxidative decarboxylation of pyruvate, producing acetylphosphate and H2O2 to support aerobic lactobacterial growth[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Pyruvate Oxidase, Microorganisms Related Antibodies
CAS No.
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Solid

Color

Light yellow to yellow

SMILES

[Pyruvate Oxidase, Microorganisms]
EC Number

1.2.3.3
Specific Activity

≥35 U/mg protein
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Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation

SDS (418 KB)

COA (241 KB)

Handling Instructions (2659 KB)
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Pyruvate Oxidase, Microorganisms Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Pyruvate Oxidase, Microorganisms9001-96-1PoxBBiochemical Assay ReagentsLactobacillus plantarum L-809flavin adenine dinucleotideEscherichia coli K-12 strain JRG3445acetyl-CoA synthetasePyruvate OxidaseMicroorganismsthiamine pyrophosphateEscherichia coli strain 191-6Escherichia coli K-12 strain JRG3980Streptococcus pneumoniaeEscherichia coli K-12 strain JRG3456Inhibitorinhibitorinhibit

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