Glutamate oxidase
Based on 1 Customer Validation
Glutamate oxidase (L-Glutamate oxidase) is a FAD-dependent oxidase with high substrate selectivity. Glutamate oxidase catalyzes the oxidative deamination of L-glutamate using O2 to produce α-ketoglutarate (α-KG), NH3, and H2O2. Glutamate oxidase is applicable to research related to the biocatalytic production of α-KG and the analytical detection of L-glutamate.
For research use only. We do not sell to patients.
- CAS No.: 39346-34-4
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Glutamate oxidase (L-Glutamate oxidase) selectively catalyzes the conversion of L-glutamate into α-ketoglutarate, NH3 and H2O2. The L-glutamate oxidase derived from Streptomyces sp. X-119-6 has a Km value of 0.21 mM for L-glutamate, and its activity toward L-aspartate is merely 0.6% of that toward L-glutamate[2].
Glutamate oxidase (1.5 U/mL; 24 h) produces 104.7 g/L α-KG with a conversion rate of 95.8% under the conditions of 250 U/mL catalase, 3 mM MnCl2, 110 g/L L-glutamate, 30 °C and pH 6.5[4].
Glutamate oxidase only converts L-glutamate among the 25 tested amino acids. The Km values of L-glutamate oxidase derived from Streptomyces endus for L-glutamate and O2 are 1.1 mM and 1.86 mM, respectively[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
≥ 5 U/mg soild
One unit is defined as the amount of enzyme that will form 1.0 μmol of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30°C.
Chemical Information
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CAS No. 39346-34-4
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Appearance Solid
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Color White to off-white
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SMILES
[Glutamate oxidase]
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Synonyms
L-Glutamate oxidase
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
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Data Sheet (266 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Zhang X, et al. Engineering of L-glutamate oxidase as the whole-cell biocatalyst for the improvement of α-ketoglutarate production. Enzyme and microbial technology. 2020 May;136:109530. [Content Brief]
[3]. Böhmer A, et al. A novel L-glutamate oxidase from Streptomyces endus. Purification and properties. Eur J Biochem. 1989;182:327-332. [Content Brief]
[4]. Niu P, et al. Enzymatic production of α-ketoglutaric acid from L-glutamic acid via L-glutamate oxidase. J Biotechnol. 2014;179:56-62. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Glutamate oxidase Related Classifications
HY-P3263 Related Classifications
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