Catalase, Aspergillus niger 

Catalase, Aspergillus niger is a key enzyme in the metabolism of H₂O₂ and reactive oxygen species (ROS), and its expression and localization is markedly altered in tumors^[1]. Free oxygen radical scavenger.

Product Information
Produced through fermentation by Aspergillus niger, it remains effective within a pH range of 4.0 to 10.0 and a temperature range of 35°C to 40°C.

Instructions
It can be dissolved using water or as specified in relevant references.
Note: When decomposing hydrogen peroxide, if the concentration of hydrogen peroxide is excessively high (≥1000 mg/L), the reaction rate of the enzyme will decrease.

Catalase, the enzyme that metabolizes H₂O₂ but also reacts with a multitude of other substrates. The active site of catalase contains an iron atom. Human catalase contains four identical subunits, each subunit containing four distinct domains and one prosthetic heme group. The four domains include: (1) a N-terminal arm which contains a distal histidine, an essential amino acid for the catalase reaction; (2) a β-barrel domain that contains eight β-barrels arranged in an antiparallel fashion with six α-helical insertions, conferring the hydrophobic core of the protein necessary for the tri-dimensional structure of the enzyme; (3) a connection domain which contains the tyrosine residue that binds the heme group; and finally (4) an α-helical domains, which is important for NADPH binding^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

9001-05-2

Solid

Off-white to light yellow

[Catalase, Aspergillus niger]

1.11.1.6

≥200000 U/g

One unit is defined as the amount of enzyme that can hydrolyze 1.0 μmole H2O2 per min at pH 7.0 and 25°C

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Glorieux C, et al. Catalase, a remarkable enzyme: targeting the oldest antioxidant enzyme to find a new cancer treatment approach. Biol Chem. 2017 Sep 26;398(10):1095-1108.  [Content Brief]