Cat. No.: HY-N0637A

(±)-Eriodictyol

(Synonyms: (±)-Huazhongilexone; Dihydroluteolin)

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(±)-Eriodictyol ((±)-Huazhongilexone; Dihydroluteolin) is an orally active TRPV1 receptor antagonist (IC₅₀=44-47 nM, rTRPV1) with antioxidant and anti-inflammatory activities. (±)-Eriodictyol effectively inhibits lipid peroxidation and the release of proinflammatory cytokines by specifically antagonizing the TRPV1 receptor and activating the Nrf2 signaling pathway. (±)-Eriodictyol reduces the levels of ICAM-1, VEGF, eNOS and TNF-α in the retina and maintains the integrity of the blood-retinal barrier. (±)-Eriodictyol alleviates oxidative stress-induced apoptosis and hyperalgesia, enhances the activity and cytotoxicity of immune cells (such as B lymphocytes, NK cells and macrophages), and increases the levels of antioxidant enzymes simultaneously. (±)-Eriodictyol can be used in the research of diabetic retinopathy, acute lung injury and various pain-related diseases.

For research use only. We do not sell to patients.

Purity: 99.9%
CAS No.: 4049-38-1
Formula: C₁₅H₁₂O₆
Molecular Weight:288.25

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

(±)-Eriodictyol Chemical Structure

CAS No.: 4049-38-1

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target

rTRPV1

All Melanocortin Receptor Isoforms :

All TRP Channel Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
B16-4A5	IC₅₀	48 μM Compound: 1	Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs by microplate reader analysis Inhibition of melanogenesis in theophylline-stimulated mouse B16-4A5 cells after 72 hrs by microplate reader analysis	[PMID: 25987378]
Neutrophil	IC₅₀	1.4 μM Compound: 5b	Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of HOCl-induced oxidation of APF after 6 mins by fluorescence assay Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of HOCl-induced oxidation of APF after 6 mins by fluorescence assay	[PMID: 23871908]
Neutrophil	IC₅₀	15.5 μM Compound: 5b	Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of superoxide anion radical-induced lucigenin oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of superoxide anion radical-induced lucigenin oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay	[PMID: 23871908]
Neutrophil	IC₅₀	18 μM Compound: 5b	Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of ROS-induced luminol oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of ROS-induced luminol oxidation incubated for 5 mins prior to PMA challenge by chemiluminescence assay	[PMID: 23871908]
Neutrophil	IC₅₀	4 μM Compound: 5b	Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of H2O2-induced oxidation of amplex red incubated for 5 mins prior to PMA challenge by fluorescence assay Inhibition of oxidative burst in PMA-stimulated human neutrophils assessed as inhibition of H2O2-induced oxidation of amplex red incubated for 5 mins prior to PMA challenge by fluorescence assay	[PMID: 23871908]

In Vitro

(±)-Eriodictyol inhibits LPS-induced inflammatory cytokine protein production and mRNA expression in C57BL/6 mouse bone marrow-derived macrophages^[2].
(±)-Eriodictyol (3-300 nM) acts as a functional TRPV1 antagonist in rat spinal cord synaptosomes, inhibiting capsaicin-induced calcium influx with an IC₅₀ of 44 nM, and does not alter baseline calcium levels on its own^[3].
(±)-Eriodictyol (1-300 μM; 30 min) has cell-free antioxidant activity, scavenging ABTS radicals with an IC₅₀ of 1.7 μM^[3].
(±)-Eriodictyol (2.5-20 μM; 48 h) dose-dependently inhibits LPS-induced NO production in murine peritoneal macrophages, reducing NO to 31.8 μM at 20 μM over 48 h^[4].
(±)-Eriodictyol (2.5-20 μM; 48 h) dose-dependently inhibits lysosomal enzyme activity in murine peritoneal macrophages, with significant suppression at 2.5, 5, 10, and 20 μM over 48 h^[4].
(±)-Eriodictyol (6.25-50 μM; 1 h) exhibits dose-dependent cellular antioxidant activity in murine splenocytes and macrophages, with EC₅₀ values of 13 μM and 14 μM, respectively, in the CAA assay^[4].
(±)-Eriodictyol (5-20 μM; 24 h) dose-dependently suppresses the production of proinflammatory cytokines TNF-α and IL-8 in high glucose-stimulated rat RGC-5 retinal ganglial cells^[5].
(±)-Eriodictyol (5-20 μM; 24 h) dose-dependently represses apoptosis in high glucose-stimulated rat RGC-5 retinal ganglial cells by modulating the expression of pro- and anti-apoptotic proteins^[5].
(±)-Eriodictyol (5-20 μM; 24 h) enhances activation of the Nrf2/HO-1 pathway in high glucose-stimulated rat RGC-5 retinal ganglial cells, which is required for its protective effects against oxidative stress, inflammation, and apoptosis^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[5]

Cell Line:

rat RGC-5 retinal ganglial cells
Concentration:

5-20 μM (pretreated, then incubated with HG); 5-20 μM (incubated with NG)
Incubation Time:

24 h
Result:

Showed no effect on RGC-5 cell viability under NG conditions.
Improved RGC-5 cell viability in a dose-dependent manner under HG conditions, with significant increases observed at all tested concentrations.

ELISA Assay^[5]

Cell Line:

rat RGC-5 retinal ganglial cells
Concentration:

5-20 μM (pretreated, then incubated with HG)
Incubation Time:

24 h
Result:

Dose-dependently reduced the HG-induced increases in TNF-α and IL-8 levels in RGC-5 cell supernatants.

Apoptosis Analysis^[5]

Cell Line:

rat RGC-5 retinal ganglial cells
Concentration:

5-20 μM (pretreated, then incubated with HG)
Incubation Time:

24 h
Result:

Dose-dependently reduced HG-induced apoptosis.
Reversed the HG-induced changes in apoptosis-related protein expression: increased bcl-2 levels and decreased bax and cleaved caspase-3 levels.

Western Blot Analysis^[5]

Cell Line:

rat RGC-5 retinal ganglial cells
Concentration:

5-20 μM (pretreated, then incubated with HG); 20 μM (pretreated, then incubated with HG for Nrf2/HO-1 Western blot quantification)
Incubation Time:

24 h
Result:

Enhanced HG-induced nuclear translocation of Nrf2 and HG-induced HO-1 protein expression in RGC-5 cells.
Knockdown of Nrf2 attenuated the protective effects of eriodictyol on HG-induced cell viability reduction, ROS production, TNF-α elevation, and apoptosis.

In Vivo

(±)-Eriodictyol (0.1-10 mg/kg; p.o.; daily; 10 days) dose-dependently reduces retinal inflammatory markers (TNF-α, eNOS) and plasma lipid peroxidation in streptozotocin-induced diabetic rats, with the 10 mg/kg dose suppressing blood-retinal barrier breakdown by 40% and lowering retinal VEGF and ICAM-1 levels^[1].
(±)-Eriodictyol (30 mg/kg; p.o.; single dose; 2 days prior to LPS exposure) significantly attenuates LPS-induced acute lung injury in female C57BL/6 mice via anti-inflammatory and antioxidative mechanisms, including improved survival, reduced inflammatory cytokine production, and activation of the Nrf2-Trx1 pathway^[2].
(±)-Eriodictyol (0.45-13.5 mg/kg; p.o.; single dose; 1 hour before capsaicin injection; 1-30 nmol/site; i.t.; single dose; 15 minutes before capsaicin injection) induces antinociception in intraplantar capsaicin-induced nociception in mice, with a maximal oral inhibition of 49% (ID₅₀=2.3 mg/kg) and maximal intrathecal inhibition of 64% (ID₅₀=2.2 nmol/site)^[3].
(±)-Eriodictyol (4.5 mg/kg; p.o.; single dose; 1 hour before intrathecal capsaicin injection) inhibits intrathecal capsaicin-induced nociception in mice by 71%^[3].
(±)-Eriodictyol (4.5 mg/kg; p.o.; single dose; 1 hour before nociception testing) fully reverses thermal hyperalgesia and reduces mechanical allodynia by 64% in CFA-induced inflammatory pain in mice^[3].
(±)-Eriodictyol (4.5 mg/kg; p.o.; single dose; 1 hour before intraplantar capsaicin injection) prevents intraplantar capsaicin-induced spinal oxidative stress in mice by preserving non-protein thiol levels and blocking 3-nitrotyrosine formation^[3].
(±)-Eriodictyol (combined with hesperidin and eriocitrin) enhances systemic antioxidant capacity and reduces markers of inflammation and oxidative stress in high-fat diet-fed C57BL/6J mice^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

albino Swiss mice (three-month-old male, 25-35 g)^[3]
Dosage:

4.5 mg/kg
Administration:

p.o.; 1 hour before intrathecal capsaicin injection; single dose
Result:

Produced a 71 ± 5% inhibition of intrathecal capsaicin-induced nociception.

Animal Model:

albino Swiss mice (three-month-old male, 25-35 g)^[3]
Dosage:

4.5 mg/kg
Administration:

p.o.; 1 hour before nociception testing; single dose
Result:

Fully reversed CFA-induced thermal hyperalgesia (100% inhibition).
Partially reduced CFA-induced mechanical allodynia (64 ± 7% inhibition).

Animal Model:

albino Swiss mice (three-month-old male, 25-35 g)^[3]
Dosage:

4.5 mg/kg
Administration:

p.o.; 1 hour before intraplantar capsaicin injection; single dose
Result:

Prevented the capsaicin-induced loss of non-protein thiols in the spinal cord.
Prevented the capsaicin-induced increase in spinal 3-NT levels.
Did not alter basal non-protein thiol or 3-NT levels in mice receiving intraplantar vehicle.

Animal Model:

albino Swiss mice (three-month-old male, 25-35 g)^[3]
Dosage:

4.5 mg/kg
Administration:

p.o.; single dose
Result:

Did not produce a significant change in rectal temperature at any measured time point compared to basal levels.

Chemical Information

CAS No. 4049-38-1
Appearance Solid
Molecular Weight 288.25
Formula C₁₅H₁₂O₆
Color White to off-white
SMILES

O=C1CC(C2=CC=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C13
Synonyms

(±)-Huazhongilexone; Dihydroluteolin
Structure Classification
- Flavonoids
- Flavones
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (346.92 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

3.4692 mL

17.3461 mL

34.6921 mL

5 mM

0.6938 mL

3.4692 mL

6.9384 mL

10 mM

0.3469 mL

1.7346 mL

3.4692 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (8.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (8.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (8.67 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (286 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Bucolo C, et al. Eriodictyol prevents early retinal and plasma abnormalities in streptozotocin-induced diabetic rats. Biochem Pharmacol. 2012;84(1):88-92. [Content Brief]

[2]. Zhu GF, et al. Eriodictyol, a plant flavonoid, attenuates LPS-induced acute lung injury through its antioxidative and anti-inflammatory activity. Exp Ther Med. 2015;10(6):2259-2266. [Content Brief]

[3]. Rossato MF, et al. Eriodictyol: a flavonoid antagonist of the TRPV1 receptor with antioxidant activity. Biochem Pharmacol. 2011;81(4):544-551. [Content Brief]

[4]. Mokdad-Bzeouich I, et al. Investigation of immunomodulatory and anti-inflammatory effects of eriodictyol through its cellular anti-oxidant activity. Cell Stress Chaperones. 2016;21(5):773-781. [Content Brief]

[5]. Lv P, et al. Eriodictyol inhibits high glucose-induced oxidative stress and inflammation in retinal ganglial cells. J Cell Biochem. 2019;120(4):5644-5651. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	3.4692 mL	17.3461 mL	34.6921 mL	86.7303 mL
	5 mM	0.6938 mL	3.4692 mL	6.9384 mL	17.3461 mL
	10 mM	0.3469 mL	1.7346 mL	3.4692 mL	8.6730 mL
	15 mM	0.2313 mL	1.1564 mL	2.3128 mL	5.7820 mL
	20 mM	0.1735 mL	0.8673 mL	1.7346 mL	4.3365 mL
	25 mM	0.1388 mL	0.6938 mL	1.3877 mL	3.4692 mL
	30 mM	0.1156 mL	0.5782 mL	1.1564 mL	2.8910 mL
	40 mM	0.0867 mL	0.4337 mL	0.8673 mL	2.1683 mL
	50 mM	0.0694 mL	0.3469 mL	0.6938 mL	1.7346 mL
	60 mM	0.0578 mL	0.2891 mL	0.5782 mL	1.4455 mL
	80 mM	0.0434 mL	0.2168 mL	0.4337 mL	1.0841 mL
	100 mM	0.0347 mL	0.1735 mL	0.3469 mL	0.8673 mL

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(±)-Eriodictyol

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All Melanocortin Receptor Isoforms

All TRP Channel Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

(±)-Eriodictyol Related Classifications

HY-N0637A Related Classifications