AACS Protein, Human (sf9, His)
AACS proteins play a key role in cellular metabolism by converting acetoacetate in the cytoplasm into acetoacetyl-CoA (by similarity). As a ketone body utilizing enzyme, AACS helps synthesize essential cellular components including cholesterol and fatty acids (by similarity). AACS Protein, Human (sf9, His) is the recombinant human-derived AACS protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
AACS proteins play a key role in cellular metabolism by converting acetoacetate in the cytoplasm into acetoacetyl-CoA (by similarity). As a ketone body utilizing enzyme, AACS helps synthesize essential cellular components including cholesterol and fatty acids (by similarity). AACS Protein, Human (sf9, His) is the recombinant human-derived AACS protein, expressed by Sf9 insect cells , with N-His labeled tag.
Background
The AACS protein is instrumental in cellular metabolism as it converts acetoacetate to acetoacetyl-CoA in the cytosol, showcasing its involvement in ketone body utilization. This enzymatic activity is integral to the synthesis of cholesterol and fatty acids, underscoring AACS's pivotal role in lipid metabolism. As a key player in the conversion of acetoacetate, a ketone body, into acetoacetyl-CoA, AACS contributes to the cellular processes that are crucial for the synthesis of essential lipids, emphasizing its significance in maintaining lipid homeostasis and supporting biosynthetic pathways for cholesterol and fatty acids (
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His
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Accession
Q86V21-1 (M1-F672)
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Molecular Construction
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N-term
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His
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AACS (M1-F672)
Accession # Q86V21-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
AACS; FLJ12389; Acetoacetyl-CoA Synthetase; Homolog Of C. Elegans Supressor Of Ras 5 (Sur-5); ACSF1; Alternative Protein AACS; Acyl-CoA Synthetase Family Member 1; Acetoacetate-CoA Ligase; SUR-5; AACS Protein; Protein Sur-5 Homolog
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AA Sequence
MSKEERPGREEILECQVMWEPDSKKNTQMDRFRAAVGAACGLALESYDDLYHWSVESYSDFWAEFWKFSGIVFSRVYDEVVDTSKGIADVPEWFKGSRLNYAENLLRHKENDRVALYIAREGKEEIVKVTFEELRQEVALFAAAMRKMGVKKGDRVVGYLPNSEHAVEAMLAAASIGAIWSSTSPDFGVNGVLDRFSQIQPKLIFSVEAVVYNGKEHNHMEKLQQVVKGLPDLKKVVVIPYVSSRENIDLSKIPNSVFLDDFLATGTSEQAPQLEFEQLPFSHPLFIMFSSGTTGAPKCMVHSAGGTLIQHLKEHLLHGNMTSSDILLCYTTVGWMMWNWMVSLLATGAAMVLYDGSPLVPTPNVLWDLVDRIGITVLVTGAKWLSVLEEKAMKPVETHSLQMLHTILSTGSPLKAQSYEYVYRCIKSSILLGSISGGTDIISCFMGHNFSLPVYKGEIQARNLGMAVEAWNEEGKAVWGESGELVCTKPIPCQPTHFWNDENGNKYRKAYFSKFPGIWAHGDYCRINPKTGGIVMLGRSDGTLNPNGVRFGSSEIYNIVESFEEVEDSLCVPQYNKYREERVILFLKMASGHAFQPDLVKRIRDAIRMGLSARHVPSLILETKGIPYTLNGKKVEVAVKQIIAGKAVEQGGAFSNPETLDLYRDIPELQGF
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Predicted Molecular Mass
77 kDa
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Molecular Weight
Approximately 60 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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