ACKR2 Protein, Human (Cell-Free, His)

ACKR2 Protein, an atypical chemokine receptor, controls chemokine levels and localization with high-affinity binding. Operating independently, ACKR2, also known as an interceptor or decoy receptor, interacts with diverse chemokines, triggering a beta-arrestin 1-dependent signaling pathway. This pathway facilitates ACKR2 relocation, enhancing efficiency in chemokine uptake and degradation. Scavenging chemokines in various tissues, ACKR2 plays a pivotal role in resolving inflammation, regulating adaptive immune responses, and influencing leukocyte interactions with lymphatic endothelial cells. ACKR2 contributes significantly to immune silencing of macrophages during inflammation resolution. ACKR2 Protein, Human (Cell-Free, His) is the recombinant human-derived ACKR2 protein, expressed by E. coli Cell-free, with N-10*His labeled tag. The total length of ACKR2 Protein, Human (Cell-Free, His) is 384 a.a., with molecular weight of 46.9 kDa.

ACKR2, an atypical chemokine receptor, orchestrates precise control over chemokine levels and localization through high-affinity chemokine binding, operating independently of classic ligand-driven signal transduction cascades. Also referred to as an interceptor, internalizing receptor, chemokine-scavenging receptor, or chemokine decoy receptor, ACKR2 serves as a receptor for a diverse array of chemokines, including CCL2, CCL3, CCL3L1, CCL4, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL22, CCL23, CCL24, SCYA2/MCP-1, SCY3/MIP-1-alpha, SCYA5/RANTES, and SCYA7/MCP-3. Upon active ligand stimulation, it triggers a beta-arrestin 1 (ARRB1)-dependent, G protein-independent signaling pathway, leading to the phosphorylation of the actin-binding protein cofilin (CFL1) through a RAC1-PAK1-LIMK1 signaling cascade. Activation of this pathway facilitates the relocation of ACKR2 from endosomal compartments to the cell membrane, enhancing its efficiency in chemokine uptake and degradation. By scavenging chemokines in tissues, lymphatic vessel surfaces, and the placenta, ACKR2 plays a pivotal role in resolving the inflammatory response and regulating adaptive immune responses. Moreover, it acts as a key regulator of inflammatory leukocyte interactions with lymphatic endothelial cells (LECs) and is essential for discerning immature/mature dendritic cells by LECs. ACKR2 also contributes significantly to immune silencing of macrophages during inflammation resolution.

Human

E. coli Cell-free

N-10*His

O00590 (M1-A384)

1238  [NCBI]

MAATASPQPLATEDADSENSSFYYYDYLDEVAFMLCRKDAVVSFGKVFLPVFYSLIFVLGLSGNLLLLMVLLRYVPRRRMVEIYLLNLAISNLLFLVTLPFWGISVAWHWVFGSFLCKMVSTLYTINFYSGIFFISCMSLDKYLEIVHAQPYHRLRTRAKSLLLATIVWAVSLAVSIPDMVFVQTHENPKGVWNCHADFGGHGTIWKLFLRFQQNLLGFLLPLLAMIFFYSRIGCVLVRLRPAGQGRALKIAAALVVAFFVLWFPYNLTLFLHTLLDLQVFGNCEVSQHLDYALQVTESIAFLHCCFSPILYAFSSHRFRQYLKAFLAAVLGWHLAPGTAQASLSSCSESSILTAQEEMTGMNDLGERQSENYPNKEDVGNKSA

46.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.