Aconitase 1/ACO1 Protein, Human (sf9, His)
Based on 1 Customer Validation
The ACO1 protein is a bifunctional iron sensor that dynamically adapts to cellular iron levels. Under conditions of iron deficiency, it is converted to mRNA binding and regulates iron-related gene expression. Aconitase 1/ACO1 Protein, Human (sf9, His) is the recombinant human-derived Aconitase 1/ACO1 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The ACO1 protein is a bifunctional iron sensor that dynamically adapts to cellular iron levels. Under conditions of iron deficiency, it is converted to mRNA binding and regulates iron-related gene expression. Aconitase 1/ACO1 Protein, Human (sf9, His) is the recombinant human-derived Aconitase 1/ACO1 protein, expressed by Sf9 insect cells , with N-His labeled tag.
Background
The Aconitase 1/ACO1 protein serves as a bifunctional iron sensor, dynamically adapting its activities based on cellular iron availability. Under conditions of iron deprivation, it undergoes a shift to mRNA binding activity, regulating the expression of genes involved in iron uptake, sequestration, and utilization. ACO1 binds to iron-responsive elements (IRES) in the untranslated regions of target mRNAs, thereby modulating translation—for instance, inhibiting the translation of ferritin and aminolevulinic acid synthase while stabilizing the mRNA of the transferrin receptor. Conversely, when cellular iron levels are elevated, ACO1 forms a 4Fe-4S cluster that precludes RNA binding activity and facilitates its aconitase activity. In this mode, ACO1 catalyzes the isomerization of citrate to isocitrate via cis-aconitate. This dual functionality underscores ACO1's crucial role in the intricate regulatory network governing cellular responses to varying iron concentrations.
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-10*His
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Accession
P21399 (M1-K889)
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Molecular Construction
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N-term
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10*His
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ACO1 (M1-K889)
Accession # P21399 -
C-term
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Protein Length
Full Length
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Synonyms
ACO1; Cytosplasmic Aconitase; Prev. IREB1; Soluble Aconitase; IRP1; Epididymis Secretory Sperm Binding Protein; Iron-Responsive Element-Binding Protein 1; Epididymis Luminal Protein 60; Cytoplasmic Aconitate Hydratase; Cytoplasmic Aconitase; Iron Regulato
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AA Sequence
MSNPFAHLAEPLDPVQPGKKFFNLNKLEDSRYGRLPFSIRVLLEAAIRNCDEFLVKKQDIENILHWNVTQHKNIEVPFKPARVILQDFTGVPAVVDFAAMRDAVKKLGGDPEKINPVCPADLVIDHSIQVDFNRRADSLQKNQDLEFERNRERFEFLKWGSQAFHNMRIIPPGSGIIHQVNLEYLARVVFDQDGYYYPDSLVGTDSHTTMIDGLGILGWGVGGIEAEAVMLGQPISMVLPQVIGYRLMGKPHPLVTSTDIVLTITKHLRQVGVVGKFVEFFGPGVAQLSIADRATIANMCPEYGATAAFFPVDEVSITYLVQTGRDEEKLKYIKKYLQAVGMFRDFNDPSQDPDFTQVVELDLKTVVPCCSGPKRPQDKVAVSDMKKDFESCLGAKQGFKGFQVAPEHHNDHKTFIYDNTEFTLAHGSVVIAAITSCTNTSNPSVMLGAGLLAKKAVDAGLNVMPYIKTSLSPGSGVVTYYLQESGVMPYLSQLGFDVVGYGCMTCIGNSGPLPEPVVEAITQGDLVAVGVLSGNRNFEGRVHPNTRANYLASPPLVIAYAIAGTIRIDFEKEPLGVNAKGQQVFLKDIWPTRDEIQAVERQYVIPGMFKEVYQKIETVNESWNALATPSDKLFFWNSKSTYIKSPPFFENLTLDLQPPKSIVDAYVLLNLGDSVTTDHISPAGNIARNSPAARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEYLPGENADALGLTGQERYTIIIPENLKPQMKVQVKLDTGKTFQAVMRFDTDVELTYFLNGGILNYMIRKMAK
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Predicted Molecular Mass
101 kDa
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Molecular Weight
Approximately 90 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris, 300 mM NaCl, pH 8.0, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (241 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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