2. Protéines recombinantes
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. ADH1 Protein, Geobacillus thermodenitrificans (His, Strep)

ADH1 Protein, Geobacillus thermodenitrificans (His, Strep)

Cat. No.: HY-P703456
Instruction de manipulation Technical Support

ADH1 Protein, Geobacillus thermodenitrificans (His, Strep) is the recombinant ADH1, expressed by E. coli, with Strep, His labeled tag.

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Description

ADH1 Protein, Geobacillus thermodenitrificans (His, Strep) is the recombinant ADH1, expressed by E. coli, with Strep, His labeled tag.
Background

ADH1 is a long-chain alkyl alcohol dehydrogenase capable of oxidizing a wide range of alkyl alcohols, from ethanol to 1-triacontanol (C2 to C30), as well as 1,3-propanediol and acetaldehyde. Its preferred substrate is ethanol, and it also oxidizes glycerol.
Species

Others
Source

E. coli
Tag

Strep;His
Accession

A4IP64 (S2-A395)
Gene ID

87620691
Protein Length

Full Length
Synonyms
Long-chain-alcohol dehydrogenase 1; Alcohol dehydrogenase 1; ADH1; Fatty alcohol oxidoreductase 1; Glycerol dehydrogenase; 1.1.1.6; adh1; Geobacillus thermodenitrificans (strain NG80-2); ADH1; Oxidoreductase; 1.1.1.192
AA Sequence

SVARIVFPPLSHVGWGALDQLVPEVKRLGAKHILVITDPMLVKIGLVDQVTSPLRQEGYSVHVYTDVVPEPPLETGEKAVAFARDGKFDLVIGVGGGSALDLAKLAAVLAVHDGSVADYLNLTGTRTLEKKGLPKILIPTTSGTGSEVTNISVLSLETTKDVVTHDYLLADVAIVDPQLTVSVPPRVTAATGIDALTHAVEAYVSVNASPTSDGLAVAAIRLISRSLRKAVANGSDKQARIDMANGSYLAGLAFFNAGVAGVHALAYPLGGQFHIAHGESNAVLLPYVMGYIRQSCTKRMADIFNALGGNSSFLSEVEASYRCVEELERFVADVGIPKTLGGFGIPESALESLTKDAVQQKRLLARSPLPLLEADIRAIYEAAFAGTIVEPHKA

Predicted Molecular Mass
45.6 kDa
Pureté

≥ 90%, as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Instruction de manipulation (2659 KB)

ADH1 Protein, Geobacillus thermodenitrificans (His, Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ADH1 Protein, Geobacillus thermodenitrificans (His, Strep)
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