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  3. Hydrolases (EC 3)
  4. ADI Protein, Metamycoplasma hominis (HEK293, His)

ADI Protein, Metamycoplasma hominis (HEK293, His)

Cat. No.: HY-P700625
Handling Instructions Technical Support

ADI Proteinas, central to the ADI pathway, catalyzes the initial step in L-arginine degradation. Specifically, it converts L-arginine to carbamoyl phosphate, initiating the pathway and playing a key role in regulating the overall metabolic breakdown of L-arginine. ADI Protein, Metamycoplasma hominis (HEK293, His) is the recombinant ADI protein, expressed by HEK293 , with C-10*His labeled tag.

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20 μg 해외재고보유
50 μg 해외재고보유
100 μg 해외재고보유
> 100 μg   견적 받기  

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  • 제품 설명

제품 설명

ADI Proteinas, central to the ADI pathway, catalyzes the initial step in L-arginine degradation. Specifically, it converts L-arginine to carbamoyl phosphate, initiating the pathway and playing a key role in regulating the overall metabolic breakdown of L-arginine. ADI Protein, Metamycoplasma hominis (HEK293, His) is the recombinant ADI protein, expressed by HEK293 , with C-10*His labeled tag.

Background

ADI, the enzyme central to the L-arginine degradation via ADI pathway, catalyzes the initial step of this amino acid degradation process. Specifically, ADI facilitates the conversion of L-arginine to carbamoyl phosphate in a crucial enzymatic reaction that marks the beginning of the pathway. This step is fundamental to the overall metabolic breakdown of L-arginine, showcasing the key role of ADI in regulating this amino acid degradation pathway.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Others

Source

HEK293

Tag

C-10*His

Accession

P41141 (S2-W409)

Gene ID

/

Molecular Construction
N-term
ADI (S2-W409)
Accession # P41141
10*His
C-term
Protein Length

Full Length of Mature Protein

Synonyms
Arginine deiminase; Arginine dihydrolase (AD); arcA
AA Sequence

SVFDSKFNGIHVYSEIGELETVLVHEPGREIDYITPARLDELLFSAILESHDARKEHQSFVKIMKDRGINVVELTDLVAETYDLASKAAKEEFIETFLEETVPVLTEANKKAVRAFLLSKPTHEMVEFMMSGITKYELGVESENELIVDPMPNLYFTRDPFASVGNGVTIHFMRYIVRRRETLFARFVFRNHPKLVKTPWYYDPAMKMPIEGGDVFIYNNETLVVGVSERTDLDTITLLAKNIKANKEVEFKRIVAINVPKWTNLMHLDTWLTMLDKNKFLYSPIANDVFKFWDYDLVNGGAEPQPQLNGLPLDKLLASIINKEPVLIPIGGAGATEMEIARETNFDGTNYLAIKPGLVIGYDRNEKTNAALKAAGITVLPFHGNQLSLGMGNARCMSMPLSRKDVKW

분자량

50kDa

Purity

≥ 90%, as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

선적

Room temperature in continental US; may vary elsewhere.

각종 서류

ADI Protein, Metamycoplasma hominis (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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상품명:
ADI Protein, Metamycoplasma hominis (HEK293, His)
Cat. No.:
HY-P700625
수량:
MCE Japan Authorized Agent: