ADI Protein, Metamycoplasma hominis (HEK293, His)
Based on 1 Customer Validation
ADI Proteinas, central to the ADI pathway, catalyzes the initial step in L-arginine degradation. Specifically, it converts L-arginine to carbamoyl phosphate, initiating the pathway and playing a key role in regulating the overall metabolic breakdown of L-arginine. ADI Protein, Metamycoplasma hominis (HEK293, His) is the recombinant ADI protein, expressed by HEK293 , with C-10*His labeled tag.
- Species: Others
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ADI Proteinas, central to the ADI pathway, catalyzes the initial step in L-arginine degradation. Specifically, it converts L-arginine to carbamoyl phosphate, initiating the pathway and playing a key role in regulating the overall metabolic breakdown of L-arginine. ADI Protein, Metamycoplasma hominis (HEK293, His) is the recombinant ADI protein, expressed by HEK293 , with C-10*His labeled tag.
Background
ADI, the enzyme central to the L-arginine degradation via ADI pathway, catalyzes the initial step of this amino acid degradation process. Specifically, ADI facilitates the conversion of L-arginine to carbamoyl phosphate in a crucial enzymatic reaction that marks the beginning of the pathway. This step is fundamental to the overall metabolic breakdown of L-arginine, showcasing the key role of ADI in regulating this amino acid degradation pathway.
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Others
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Source HEK293
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Tag C-10*His
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Accession
P41141 (S2-W409)
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Gene ID/
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Molecular Construction
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N-term
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ADI (S2-W409)
Accession # P41141 -
10*His
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C-term
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Protein Length
Full Length
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Synonyms
Arginine deiminase; EC:3.5.3.6; ADI; Arginine dihydrolase (AD); arcA
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AA Sequence
SVFDSKFNGIHVYSEIGELETVLVHEPGREIDYITPARLDELLFSAILESHDARKEHQSFVKIMKDRGINVVELTDLVAETYDLASKAAKEEFIETFLEETVPVLTEANKKAVRAFLLSKPTHEMVEFMMSGITKYELGVESENELIVDPMPNLYFTRDPFASVGNGVTIHFMRYIVRRRETLFARFVFRNHPKLVKTPWYYDPAMKMPIEGGDVFIYNNETLVVGVSERTDLDTITLLAKNIKANKEVEFKRIVAINVPKWTNLMHLDTWLTMLDKNKFLYSPIANDVFKFWDYDLVNGGAEPQPQLNGLPLDKLLASIINKEPVLIPIGGAGATEMEIARETNFDGTNYLAIKPGLVIGYDRNEKTNAALKAAGITVLPFHGNQLSLGMGNARCMSMPLSRKDVKW
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Predicted Molecular Mass
48 kDa
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Molecular Weight
Approximately 50 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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