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  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. ADI Protein, Metamycoplasma hominis (HEK293, His)

ADI Protein, Metamycoplasma hominis (HEK293, His)

Cat. No.: HY-P700625
COA Handling Instructions

ADI Proteinas, central to the ADI pathway, catalyzes the initial step in L-arginine degradation. Specifically, it converts L-arginine to carbamoyl phosphate, initiating the pathway and playing a key role in regulating the overall metabolic breakdown of L-arginine. ADI Protein, Metamycoplasma hominis (HEK293, His) is the recombinant ADI protein, expressed by HEK293, with C-10*His labeled tag. The total length of ADI Protein, Metamycoplasma hominis (HEK293, His) is 408 a.a., with molecular weight of 50 kDa.

For research use only. We do not sell to patients.

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Description

ADI Proteinas, central to the ADI pathway, catalyzes the initial step in L-arginine degradation. Specifically, it converts L-arginine to carbamoyl phosphate, initiating the pathway and playing a key role in regulating the overall metabolic breakdown of L-arginine. ADI Protein, Metamycoplasma hominis (HEK293, His) is the recombinant ADI protein, expressed by HEK293, with C-10*His labeled tag. The total length of ADI Protein, Metamycoplasma hominis (HEK293, His) is 408 a.a., with molecular weight of 50 kDa.

Background

ADI, the enzyme central to the L-arginine degradation via ADI pathway, catalyzes the initial step of this amino acid degradation process. Specifically, ADI facilitates the conversion of L-arginine to carbamoyl phosphate in a crucial enzymatic reaction that marks the beginning of the pathway. This step is fundamental to the overall metabolic breakdown of L-arginine, showcasing the key role of ADI in regulating this amino acid degradation pathway.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Others

Source

HEK293

Tag

C-10*His

Accession

P41141 (S2-W409)

Gene ID

/

Molecular Construction
N-term
ADI (S2-W409)
Accession # P41141
10*His
C-term
Synonyms
Arginine deiminase; Arginine dihydrolase (AD); arcA
AA Sequence

SVFDSKFNGIHVYSEIGELETVLVHEPGREIDYITPARLDELLFSAILESHDARKEHQSFVKIMKDRGINVVELTDLVAETYDLASKAAKEEFIETFLEETVPVLTEANKKAVRAFLLSKPTHEMVEFMMSGITKYELGVESENELIVDPMPNLYFTRDPFASVGNGVTIHFMRYIVRRRETLFARFVFRNHPKLVKTPWYYDPAMKMPIEGGDVFIYNNETLVVGVSERTDLDTITLLAKNIKANKEVEFKRIVAINVPKWTNLMHLDTWLTMLDKNKFLYSPIANDVFKFWDYDLVNGGAEPQPQLNGLPLDKLLASIINKEPVLIPIGGAGATEMEIARETNFDGTNYLAIKPGLVIGYDRNEKTNAALKAAGITVLPFHGNQLSLGMGNARCMSMPLSRKDVKW

Molecular Weight

50kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ADI Protein, Metamycoplasma hominis (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
ADI Protein, Metamycoplasma hominis (HEK293, His)
Cat. No.:
HY-P700625
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