Adiponectin/Acrp30 Protein, Mouse (227a.a)

Adiponectin/Acrp30 Protein, Mouse (227a.a) suppresses endothelial inflammatory response and vascular smooth muscle cell proliferation, as well as macrophage-to-foam cell transformation.

Recombinant Mouse Adiponectin promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity, and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin is also an anti-inflammatory agent; it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease.

1.The ED₅₀ is <5 μg/mL as measured by M1 cells, corresponding to a specific activity of >2.0 × 10² units/mg.
2. Measured by its ability to induce TIMP-1 secretion by RAW264.7 mouse macrophages cell. The ED₅₀ for this effect is 1.841 μg/mL, Corresponding to a specific activity is 543.183 U/mg.

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  Measured by its ability to induce TIMP-1 secretion by RAW264.7 mouse macrophages cell. The ED₅₀ for this effect is 1.841 μg/mL, Corresponding to a specific activity is 543.183 U/mg.

Mouse

E. coli

Tag Free

Q60994 (V21-N247)

11450  [NCBI]

Full Length of Mature Protein

VTTTEELAPALVPPPKGTCAGWMAGIPGHPGHNGTPGRDGRDGTPGEKGEKGDAGLLGPKGETGDVGMTGAEGPRGFPGTPGRKGEPGEAAYVYRSAFSVGLETRVTVPNVPIRFTKIFYNQQNHYDGSTGKFYCNIPGLYYFSYHITVYMKDVKVSLFKKDKAVLFTYDQYQEKNVDQASGSVLLHLEVGDQVWLQVYGDGDHNGLYADNVNDSTFTGFLLYHDTN

Approximately 26 kDa

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of PBS.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wilcox RA, et al. Provision of antigen and CD137 signaling breaks immunological ignorance, promoting regression of poorly immunogenic tumors. J Clin Invest. 2002 Mar;109(5):651-9.  [Content Brief]