ADRB2 Protein, Human (G16R, E27Q, Cell-Free, His, SUMO)
Based on 1 Customer Validation
ADRB2 protein is an important member of the β-adrenergic receptor family and mediates catecholamine-induced adenylyl cyclase activation through G protein action. It has 30 times greater affinity for epinephrine and plays a crucial role in cellular responses to sympathetic nervous system stimulation. ADRB2 Protein, Human (Cell-Free, His, SUMO) is the recombinant human-derived ADRB2 protein, expressed by E. coli Cell-free, with N-6*His, N-SUMO labeled tag with mutatant of G16R, E27Q.
- Species: Human
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ADRB2 protein is an important member of the β-adrenergic receptor family and mediates catecholamine-induced adenylyl cyclase activation through G protein action. It has 30 times greater affinity for epinephrine and plays a crucial role in cellular responses to sympathetic nervous system stimulation. ADRB2 Protein, Human (Cell-Free, His, SUMO) is the recombinant human-derived ADRB2 protein, expressed by E. coli Cell-free, with N-6*His, N-SUMO labeled tag with mutatant of G16R, E27Q.
Background
The ADRB2 Protein, belonging to the beta-adrenergic receptor family, plays a pivotal role in mediating catecholamine-induced activation of adenylate cyclase through G protein action. With approximately 30-fold higher affinity for epinephrine compared to norepinephrine, ADRB2 is a key player in cellular responses to sympathetic nervous system stimulation. The protein engages in various molecular interactions, binding to NHERF1, GPRASP1, ARRB1, ARRB2, SRC, USP20, USP33, VHL, EGLN3, SNX27, CNIH4, ARRDC3, NEDD4, and MARCHF2. These interactions contribute to the intricate regulation of ADRB2 function, including its ubiquitination, degradation, and recycling processes, underscoring its importance in modulating cellular responses to adrenergic signaling.
Technical Parameters
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Species Human
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Source E. coli Cell-free
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Tag N-6*His;N-SUMO
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Accession
P07550 (M1-L413, G16R, E27Q)
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Gene ID154
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Molecular Construction
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N-term
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6*His-SUMO
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ADRB2 (M1-L413, G16R, E27Q)
Accession # P07550 -
C-term
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Protein Length
Full Length
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Synonyms
ADRB2; ARB2; Prev. ADRB2R; Adrenergic, Beta-2-, Receptor, Surface; B2AR; Adrenoceptor Beta 2, Surface; Beta-2 Adrenergic Receptor; Adrenoceptor Beta 2 Surface; Beta-2 Adrenoreceptor; Catecholamine Receptor; Beta-2 Adrenoceptor; ADRB2 Protein; ADRBR; BETA2AR; Adrenoceptor Beta 2; BAR
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AA Sequence
MGQPGNGSAFLLAPNRSHAPDHDVTQQRDEVWVVGMGIVMSLIVLAIVFGNVLVITAIAKFERLQTVTNYFITSLACADLVMGLAVVPFGAAHILMKMWTFGNFWCEFWTSIDVLCVTASIETLCVIAVDRYFAITSPFKYQSLLTKNKARVIILMVWIVSGLTSFLPIQMHWYRATHQEAINCYANETCCDFFTNQAYAIASSIVSFYVPLVIMVFVYSRVFQEAKRQLQKIDKSEGRFHVQNLSQVEQDGRTGHGLRRSSKFCLKEHKALKTLGIIMGTFTLCWLPFFIVNIVHVIQDNLIRKEVYILLNWIGYVNSGFNPLIYCRSPDFRIAFQELLCLRRSSLKAYGNGYSSNGNTGEQSGYHVEQEKENKLLCEDLPGTEDFVGHQGTVPSDNIDSQGRNCSTNDSLL
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Predicted Molecular Mass
62.5 kDa
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Molecular Weight
Approximately 65 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.15 M NaCl, 0.05% FOS12, 6% trehalose, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij78, 6% trehalose, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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