1. Recombinant Proteins
  2. Immune Checkpoint Proteins
  3. Inhibitory checkpoint molecules
  4. CD160 & HVEM
  5. ALCAM/CD166 Protein, Cynomolgus (HEK293, His)

ALCAM/CD166 Protein, Cynomolgus (HEK293, His)

Cat. No.: HY-P76777
Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
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ALCAM/CD166 Protein, Cynomolgus (HEK293, His) Related Classifications

  • Technical Parameters

  • Properties

  • Documentation

Species

Cynomolgus

Source

HEK 293

Tag

His

Accession

G7NZQ8 (M1-A526)

Gene ID

/

Synonyms
ALCAM; CD166 antigen; Activated leukocyte cell adhesion molecule; MEMD
AA Sequence

Molecular Weight

65-68 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Biological Activity
Immobilized cynomolgus ALCAM-His at 10 μg/mL (100 μL/well) can bind mouse CD6-Fc and the EC50 is 0.07-0.15 μg/mL.
Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
ALCAM/CD166 Protein, Cynomolgus (HEK293, His)
Cat. No.:
HY-P76777
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