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ALCAM/CD166 Protein, Cynomolgus (HEK293, His)

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ALCAM/CD166 Protein is a glycoprotein involved in homotypic and heterotypic cell adhesion. ALCAM/CD166 has emerged as a modulator of cancer progression, mainly by controlling cell proliferation, adhesion, migration and invasion. ALCAM/CD166 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived ALCAM/CD166 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Cynomolgus
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for ALCAM/CD166 Protein, Cynomolgus (HEK293, His)
  • Experimental Validation Results for ALCAM/CD166 Protein, Cynomolgus (HEK293, His)
  • ALCAM/CD166 Protein, Cynomolgus (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Use of MCE Products

Biological Activity

Description

ALCAM/CD166 Protein is a glycoprotein involved in homotypic and heterotypic cell adhesion. ALCAM/CD166 has emerged as a modulator of cancer progression, mainly by controlling cell proliferation, adhesion, migration and invasion[1]. ALCAM/CD166 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived ALCAM/CD166 protein, expressed by HEK293 , with C-His labeled tag.

Background

Activated Leukocyte Cell Adhesion Molecule (ALCAM) or cluster of differentiation 166 (CD166) is a cell surface glycoprotein structurally related to the immunoglobulin superfamily molecules, initially identified on activated leukocytes. ALCAM is also found in epithelial cells, vascular endothelial cells, neurons, myeloid progenitors, hematopoietic stem cells, mesenchymal stem cells, bone marrow stromal cells, tumor cells and cancer stem cells. ALCAM can be proteolytically cleaved at the cell surface by metalloproteases, which generate shedding of its ectodomain. In normal physiology, ALCAM has been shown to modulate cell-cell interactions with lymphocytes as well as epithelial and endothelial cells. Besides cell adhesion, other ALCAM functions have been described such as angiogenesis, transmigration of monocytes, leukocyte intravasation across blood brain barrier, T cell activation, hematopoiesis, neurite extension, osteogenesis, and embryonic implantation in the uterus[1].

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey ALCAM/CD166 is immobilized at 1 µg/mL (100 µL/well) can bind Biotinylated Recombinant Human CD6. The ED50 for this effect is 102.3 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey ALCAM/CD166 is immobilized at 1 µg/mL (100 µL/well) can bind Biotinylated Recombinant Human CD6. The ED50 for this effect is 102.3 ng/mL.

Technical Parameters

  • Species Cynomolgus
  • Source HEK293
  • Tag C-His
  • Accession

    G7NZQ8 (W28-A526)

  • Gene ID
    /
  • Molecular Construction
    • N-term
    • ALCAM (W28-A526)
      Accession # G7NZQ8
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    ALCAM; CD166 Antigen; Activated Leukocyte Cell Adhesion Molecule; CD166; MEMD; Activated Leucocyte Cell Adhesion Molecule

  • AA Sequence

    WYTVNSAYGDTIIMPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLSLSENYTLSISNARISDEKRFVCMLVTEDNVFEAPTVVKVFKQPSKPEIVSKALFLETEQLKKLGDCISEDSYPDGNITWYRNGKVLHPLEGAVVIIFKKEMDPVTQLYTMTSTLEYKTTKADIQMPFTCSVTYYGPSGQKTVHSEQAVFDIYYPTEQVTIQVLPPKTAIKEGDNVTLKCLGNGNPPPEEFLFYLPGQPEGIRSSNTYTLTDVRRNATGDYKCSLIDKKSMIASTAITVHYLDLSLNPSGEVTRQIGDALPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEENVTLTCTAENQLERTVNSLNVSAISIPEHDEADEISDENREKVNDQA

  • Molecular Weight

    Approximately 65-68 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Fátima Ferragut, et al. ALCAM/CD166: A pleiotropic mediator of cell adhesion, stemness and cancer progression. Cytokine Growth Factor Rev. 2021 Oct:61:27-37. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey ALCAM/CD166 is immobilized at 1 µg/mL (100 µL/well) can bind Biotinylated Recombinant Human CD6. The ED50 for this effect is 102.3 ng/mL.

  • ALCAM/CD166 Protein, Cynomolgus (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for ALCAM/CD166 Protein, Cynomolgus (HEK293, His)
  • Experimental Validation Results for ALCAM/CD166 Protein, Cynomolgus (HEK293, His)
  • ALCAM/CD166 Protein, Cynomolgus (HEK293, His) Product Image