ALCAM/CD166 Protein, Rat (HEK293, His)

ALCAM/CD166 protein is a multifunctional cell adhesion molecule involved in various cell-cell interactions. It promotes T cell activation and immune synapse formation by interacting with CD6. ALCAM/CD166 Protein, Rat (HEK293, His) is the recombinant rat-derived ALCAM/CD166 protein, expressed by HEK293 , with C-His labeled tag.

ALCAM/CD166 Protein is a cell adhesion molecule that plays multiple roles in cell-cell interactions. It mediates both heterotypic contacts through its interaction with CD6 and homotypic contacts. Its interaction with CD6 promotes T-cell activation, proliferation, and the formation of the immunological synapse. ALCAM also mediates homotypic interactions with ALCAM-expressing cells and facilitates the attachment of dendritic cells to endothelial cells. Furthermore, it inhibits endothelial cell migration, promotes endothelial tube formation, and is necessary for the proper organization of the lymph vessel network. ALCAM is also involved in hematopoiesis, as it is required for normal numbers of hematopoietic stem cells in the bone marrow and hematopoietic stem cell engraftment. Additionally, it promotes osteoblast proliferation and differentiation, as well as neurite extension, axon growth, and axon guidance. ALCAM facilitates outgrowth and pathfinding for retinal ganglion cell axons. It forms homodimers and interacts with CD6, with both interactions occurring in the extracellular domain. However, homodimerization and CD6 interaction cannot happen simultaneously, and the affinity for CD6 is higher than for self-association. Finally, ALCAM can interact with LGALS1 and LGALS3, but this interaction inhibits its interaction with CD6 and involves the glycosylated extracellular domain.

Measured by its ability to block adhesion of HuT78 human cutaneous T cell lymphoma cells to immobilized Recombinant Human CD6. Recombinant Rat ALCAM/CD166 at 10 µg/mL blocked 81.21% of HUT78 cell adhesionto immobilized recombinant human CD6 (10 µg/mL, 100 µL/well).

Rat

HEK293

C-His

O35112 (W28-K527)

79559  [NCBI]

Extracellular Domain

WYTVNSAYGDTIVMPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLSLSENYTLSINNAKISDEKRFVCMLVTEDNVFEAPTLVKVFKQPSKPEIVNRAAFLETEQLKKLGDCISRDSYPDGNITWYRNGKVLQPVDGEVSILFKKEIDPGTQLYTMTSSLEYKTTKSDIQMPFTCSVTYYGPSGQKTIYSEQAIFDIYYPTEQVTIQVLPPKNAIKEGDNITLQCLGNGNPPPEEFMFYLPGQAEGIRSSNTYTLTDVRRNATGDYKCSLIDQRNMAASTTITVHYLDLSLNPSGEVTKQIGDTLPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEENVTLTCTAENQLERTVNSLNVSAISIPEHDEADDISDENREKVNDQAK

68-95 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.