1. Recombinant Proteins
  2. CD Antigens Receptor Proteins
  3. Macrophage CD Proteins Stem Cell CD Proteins Epithelial cell CD Proteins Cell Adhesion Molecules (CAMs)
  4. ALCAM/CD166 Immunoglobulin-like Cell Adhesion Molecules
  5. Activated Leukocyte Cell Adhesion Molecule (ALCAM)
  6. ALCAM/CD166 Protein, Rat (HEK293, His)

ALCAM/CD166 Protein, Rat (HEK293, His)

Cat. No.: HY-P75620
COA Handling Instructions

ALCAM/CD166 protein is a multifunctional cell adhesion molecule involved in various cell-cell interactions. It promotes T cell activation and immune synapse formation by interacting with CD6. ALCAM/CD166 Protein, Rat (HEK293, His) is the recombinant rat-derived ALCAM/CD166 protein, expressed by HEK293 , with C-His labeled tag. The total length of ALCAM/CD166 Protein, Rat (HEK293, His) is 500 a.a., with molecular weight of 68-95 kDa.

For research use only. We do not sell to patients.

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Description

ALCAM/CD166 protein is a multifunctional cell adhesion molecule involved in various cell-cell interactions. It promotes T cell activation and immune synapse formation by interacting with CD6. ALCAM/CD166 Protein, Rat (HEK293, His) is the recombinant rat-derived ALCAM/CD166 protein, expressed by HEK293 , with C-His labeled tag. The total length of ALCAM/CD166 Protein, Rat (HEK293, His) is 500 a.a., with molecular weight of 68-95 kDa.

Background

ALCAM/CD166 Protein is a cell adhesion molecule that plays multiple roles in cell-cell interactions. It mediates both heterotypic contacts through its interaction with CD6 and homotypic contacts. Its interaction with CD6 promotes T-cell activation, proliferation, and the formation of the immunological synapse. ALCAM also mediates homotypic interactions with ALCAM-expressing cells and facilitates the attachment of dendritic cells to endothelial cells. Furthermore, it inhibits endothelial cell migration, promotes endothelial tube formation, and is necessary for the proper organization of the lymph vessel network. ALCAM is also involved in hematopoiesis, as it is required for normal numbers of hematopoietic stem cells in the bone marrow and hematopoietic stem cell engraftment. Additionally, it promotes osteoblast proliferation and differentiation, as well as neurite extension, axon growth, and axon guidance. ALCAM facilitates outgrowth and pathfinding for retinal ganglion cell axons. It forms homodimers and interacts with CD6, with both interactions occurring in the extracellular domain. However, homodimerization and CD6 interaction cannot happen simultaneously, and the affinity for CD6 is higher than for self-association. Finally, ALCAM can interact with LGALS1 and LGALS3, but this interaction inhibits its interaction with CD6 and involves the glycosylated extracellular domain.

Biological Activity

Measured by its ability to block adhesion of HuT78 human cutaneous T cell lymphoma cells to immobilized Recombinant Human CD6. Recombinant Rat ALCAM/CD166 at 10 µg/mL blocked 81.21% of HUT78 cell adhesionto immobilized recombinant human CD6 (10 µg/mL, 100 µL/well).

Species

Rat

Source

HEK293

Tag

C-His

Accession

O35112 (W28-K527)

Gene ID

79559  [NCBI]

Molecular Construction
N-term
ALCAM (W28-K527)
Accession # O35112
His
C-term
Synonyms
CD166 antigen; Activated leukocyte cell adhesion molecule; HB2; KG-CAM; ALCAM
AA Sequence

WYTVNSAYGDTIVMPCRLDVPQNLMFGKWKYEKPDGSPVFIAFRSSTKKSVQYDDVPEYKDRLSLSENYTLSINNAKISDEKRFVCMLVTEDNVFEAPTLVKVFKQPSKPEIVNRAAFLETEQLKKLGDCISRDSYPDGNITWYRNGKVLQPVDGEVSILFKKEIDPGTQLYTMTSSLEYKTTKSDIQMPFTCSVTYYGPSGQKTIYSEQAIFDIYYPTEQVTIQVLPPKNAIKEGDNITLQCLGNGNPPPEEFMFYLPGQAEGIRSSNTYTLTDVRRNATGDYKCSLIDQRNMAASTTITVHYLDLSLNPSGEVTKQIGDTLPVSCTISASRNATVVWMKDNIRLRSSPSFSSLHYQDAGNYVCETALQEVEGLKKRESLTLIVEGKPQIKMTKKTDPSGLSKTIICHVEGFPKPAIQWTITGSGSVINQTEESPYINGRYYSKIIISPEENVTLTCTAENQLERTVNSLNVSAISIPEHDEADDISDENREKVNDQAK

Molecular Weight

68-95 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ALCAM/CD166 Protein, Rat (HEK293, His)
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HY-P75620
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