Alkaline Phosphatase/ALPG Protein, Rat (HEK293, His)

Alkaline Phosphatase/ALPG Protein, Rat (HEK293, His) is the recombinant rat-derived Alkaline Phosphatase/ALPG, expressed by HEK293, with C-His labeled tag.

The Alkaline Phosphatase/ALPG protein is an enzyme that possesses the capability to hydrolyze a range of phosphate compounds. It exhibits the ability to break down various phosphate molecules through its enzymatic activity. This protein acts as an alkaline phosphatase, catalyzing the hydrolysis of phosphate esters in an alkaline environment. Its enzymatic activity allows it to cleave phosphate groups from molecules such as nucleotides, proteins, and phospholipids, rendering them inactive. This versatile enzyme plays a vital role in various physiological processes, including bone mineralization, cellular signaling, and metabolism.

Rat

HEK293

C-8*His

F1M8U7 (V22-P510)

/

Partial

VIPVEEENPAFWNQKAKEALDVAKKLKPIRTSAKNLIIFLGDGMGVPTVTATRILKGQLGGHLGPETPLAMDHFPFTALSKTYNVDRQVPDSAGTATAYLCGVKANYKTIGVSAAARFNQCNSTFGNEVFSVMHRAKKAGKSVGVVTTTSVQHASPAGTYAHTVNCDWYSDAHMATAALQEGCKDIAMQLISNMDIDVILGGGRKFMFPKGTPDPEYSGNRAQSGTRLDGQNLVQKWLAKHQGARYVWNRTELIQASQDSAVTHLMGLFEPNDMKYDIYRDPTQDPSLAEMTEVAVHLLSRNPKGFYLFVEGGHIDHGHHESIAYRALTEAVMFDSAVDKANKLTSEKDTMILVTADHSHVFSFGGYVPRGTSIFGLGASFNALDGRPFTSILYGNGPGYKLEKGTRPDVTDKESRDPKYRQQAAVPLSSETHSGEDVAIFARGPQAHLVHGVQEQNYIAHVMAFAGCLEPYTDCGLAPPAGRSSAVSP

55-70 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.