alpha Actinin 4/ACTN4 Protein, Human (HEK293, C-His)

Alpha-actinin 4 is a member of the spectrin gene superfamily and binds to actin in a variety of cell types. It localizes to microfilament bundles, adherens junctions, and Z-disks, promoting actin binding to membranes. alpha Actinin 4 (ACTN4) Protein,Human (HEK293,C-His) is the recombinant human-derived alpha Actinin 4, expressed by HEK293 , with C-10*His labeled tag.

Alpha Actinin 4, a member of the spectrin gene superfamily, plays a multifaceted role as an actin-binding protein in various cell types. The nonmuscle isoform is distributed along microfilament bundles and adherens-type junctions, facilitating actin-membrane binding in nonmuscle cells. Conversely, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, anchoring myofibrillar actin filaments. In the cytoplasm, this gene encodes a nonmuscle alpha actinin isoform, believed to be involved in metastatic processes. Notably, mutations in this gene have been linked to focal and segmental glomerulosclerosis, highlighting its significance in both cellular architecture and disease pathology. With ubiquitous expression observed in tissues such as placenta (RPKM 105.3), kidney (RPKM 104.8), and 25 other tissues, Alpha Actinin 4 emerges as a critical player in cellular dynamics and disease-associated mechanisms.

Human

HEK293

C-10*His

NP_004915 (M1-L911)

81

Full Length

MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDALGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTALYGESDL

Approximately 106-120 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.