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alpha Actinin 4/ACTN4 Protein, Human (HEK293, C-His)

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Based on 1 Customer Validation

Alpha-actinin 4 is a member of the spectrin gene superfamily and binds to actin in a variety of cell types. It localizes to microfilament bundles, adherens junctions, and Z-disks, promoting actin binding to membranes. alpha Actinin 4 (ACTN4) Protein,Human (HEK293,C-His) is the recombinant human-derived alpha Actinin 4, expressed by HEK293 , with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for alpha Actinin 4/ACTN4 Protein, Human (HEK293, C-His)
  • alpha Actinin 4/ACTN4 Protein, Human (HEK293, C-His) Product Image
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609-228-6898
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Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Alpha-actinin 4 is a member of the spectrin gene superfamily and binds to actin in a variety of cell types. It localizes to microfilament bundles, adherens junctions, and Z-disks, promoting actin binding to membranes. alpha Actinin 4 (ACTN4) Protein,Human (HEK293,C-His) is the recombinant human-derived alpha Actinin 4, expressed by HEK293 , with C-10*His labeled tag.

Background

Alpha Actinin 4, a member of the spectrin gene superfamily, plays a multifaceted role as an actin-binding protein in various cell types. The nonmuscle isoform is distributed along microfilament bundles and adherens-type junctions, facilitating actin-membrane binding in nonmuscle cells. Conversely, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, anchoring myofibrillar actin filaments. In the cytoplasm, this gene encodes a nonmuscle alpha actinin isoform, believed to be involved in metastatic processes. Notably, mutations in this gene have been linked to focal and segmental glomerulosclerosis, highlighting its significance in both cellular architecture and disease pathology. With ubiquitous expression observed in tissues such as placenta (RPKM 105.3), kidney (RPKM 104.8), and 25 other tissues, Alpha Actinin 4 emerges as a critical player in cellular dynamics and disease-associated mechanisms.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    NP_004915 (M1-L911)

  • Gene ID
    81
  • Molecular Construction
    • N-term
    • ACTN4 (M1-L911)
      Accession # NP_004915
    • 10*His
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    ACTN4; Actinin Alpha 4 Isoform 3; Prev. FSGS1; Actinin Alpha 4 Isoform 2; Focal Segmental Glomerulosclerosis 1; Actinin Alpha 4 Isoform 1; Non-Muscle Alpha-Actinin 4; ACTN4 Protein; Alpha-Actinin-4; ACTININ-4; Actinin, Alpha 4, Isoform CRA_a; FSGS; Actini

  • AA Sequence

    MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDALGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTALYGESDL

  • Predicted Molecular Mass

    106.2 kDa

  • Molecular Weight

    Approximately 106-120 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • alpha Actinin 4/ACTN4 Protein, Human (HEK293, C-His) Product Image
    1/16
  • Experimental Validation Results for alpha Actinin 4/ACTN4 Protein, Human (HEK293, C-His)
  • alpha Actinin 4/ACTN4 Protein, Human (HEK293, C-His) Product Image