alpha Actinin 4/ACTN4 Protein, Human (HEK293, C-His)

Alpha Actinin 4, a spectrin gene superfamily member, binds actin in various cell types. It localizes to microfilament bundles, adherens-type junctions, and Z-discs, facilitating actin-membrane binding. This gene also encodes a nonmuscle isoform involved in metastasis. Mutations in this gene are linked to glomerulosclerosis. With broad expression in tissues like placenta (RPKM 105.3) and kidney (RPKM 104.8), Alpha Actinin 4 is crucial in cellular dynamics and disease mechanisms. alpha Actinin 4 (ACTN4) Protein,Human (HEK293,C-His) is the recombinant human-derived alpha Actinin 4, expressed by HEK293 , with C-10*His labeled tag. The total length of alpha Actinin 4 (ACTN4) Protein,Human (HEK293,C-His) is 911 a.a., with molecular weight of ~106-120 kDa.

Alpha Actinin 4, a member of the spectrin gene superfamily, plays a multifaceted role as an actin-binding protein in various cell types. The nonmuscle isoform is distributed along microfilament bundles and adherens-type junctions, facilitating actin-membrane binding in nonmuscle cells. Conversely, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, anchoring myofibrillar actin filaments. In the cytoplasm, this gene encodes a nonmuscle alpha actinin isoform, believed to be involved in metastatic processes. Notably, mutations in this gene have been linked to focal and segmental glomerulosclerosis, highlighting its significance in both cellular architecture and disease pathology. With ubiquitous expression observed in tissues such as placenta (RPKM 105.3), kidney (RPKM 104.8), and 25 other tissues, Alpha Actinin 4 emerges as a critical player in cellular dynamics and disease-associated mechanisms.

Human

HEK293

C-10*His

NP_004915 (M1-L911)

81

MVDYHAANQSYQYGPSSAGNGAGGGGSMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDALGSLTHSRREALEKTEKQLEAIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTTVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANVVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGEAEFNRIMSLVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAVPGALDYKSFSTALYGESDL

Approximately 106-120 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.