ANKFY1 Protein, Human (sf9, His, StrepⅡ)
ANKFY1 is a proposed Rab5 effector that critically participates in endosomal dynamics by binding to PI(3)P, promoting homotypic early endosomal fusion and participating in heterotypic fusion. It plays a crucial role in macropinocytosis, is dependent on Rab5-GTP, and is essential for the correct localization of activated tyrosine kinase receptors such as PDGFRB. ANKFY1 Protein, Human (sf9, His, Strep) is the recombinant human-derived ANKFY1 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
ANKFY1 is a proposed Rab5 effector that critically participates in endosomal dynamics by binding to PI(3)P, promoting homotypic early endosomal fusion and participating in heterotypic fusion. It plays a crucial role in macropinocytosis, is dependent on Rab5-GTP, and is essential for the correct localization of activated tyrosine kinase receptors such as PDGFRB. ANKFY1 Protein, Human (sf9, His, Strep) is the recombinant human-derived ANKFY1 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.
Background
ANKFY1, a proposed effector of Rab5, plays a crucial role in endosomal dynamics by binding to phosphatidylinositol 3-phosphate (PI(3)P) and facilitating homotypic early endosome fusion. It also participates, albeit to a lesser extent, in the heterotypic fusion of clathrin-coated vesicles with early endosomes. ANKFY1 is intricately involved in macropinocytosis, a process dependent on Rab5-GTP. Additionally, ANKFY1 is essential for the correct endosomal localization and internalization of activated tyrosine kinase receptors, such as PDGFRB. Notably, it regulates the subcellular localization of the retromer complex in an EHD1-dependent manner, impacting endosome-to-Golgi transport and biosynthetic transport to late endosomes and lysosomes. Interactions with key proteins like RAB5A, RHOD, EHD1, and VPS26A underscore ANKFY1's role in coordinating various aspects of endosomal and retrograde transport processes.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-StrepⅡ;N-8*His
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Accession
Q9P2R3 (A2-S1169)
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Gene ID51479
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Molecular Construction
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N-term
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8*His-StrepⅡ
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ANKFY1 (A2-S1169)
Accession # Q9P2R3 -
C-term
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Protein Length
Full Length
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Synonyms
ANKFY1; BTBD23; Ankyrin Repeat And FYVE Domain Containing 1; Rab5-Binding And Ankyrin Repeats-Containing Protein; ANKHZN; Ankyrin Repeat And FYVE Domain-Containing Protein 1; Rabankyrin-5; Ankyrin Repeats Hooked To A Zinc Finger Motif; KIAA1255; Ankyrin R
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AA Sequence
AEEEVAKLEKHLMLLRQEYVKLQKKLAETEKRCALLAAQANKESSSESFISRLLAIVADLYEQEQYSDLKIKVGDRHISAHKFVLAARSDSWSLANLSSTKELDLSDANPEVTMTMLRWIYTDELEFREDDVFLTELMKLANRFQLQLLRERCEKGVMSLVNVRNCIRFYQTAEELNASTLMNYCAEIIASHWDDLRKEDFSSMSAQLLYKMIKSKTEYPLHKAIKVEREDVVFLYLIEMDSQLPGKLNEADHNGDLALDLALSRRLESIATTLVSHKADVDMVDKSGWSLLHKGIQRGDLFAATFLIKNGAFVNAATLGAQETPLHLVALYSSKKHSADVMSEMAQIAEALLQAGANPNMQDSKGRTPLHVSIMAGNEYVFSQLLQCKQLDLELKDHEGSTALWLAVQHITVSSDQSVNPFEDVPVVNGTSFDENSFAARLIQRGSHTDAPDTATGNCLLQRAAGAGNEAAALFLATNGAHVNHRNKWGETPLHTACRHGLANLTAELLQQGANPNLQTEEALPLPKEAASLTSLADSVHLQTPLHMAIAYNHPDVVSVILEQKANALHATNNLQIIPDFSLKDSRDQTVLGLALWTGMHTIAAQLLGSGAAINDTMSDGQTLLHMAIQRQDSKSALFLLEHQADINVRTQDGETALQLAIRNQLPLVVDAICTRGADMSVPDEKGNPPLWLALANNLEDIASTLVRHGCDATCWGPGPGGCLQTLLHRAIDENNEPTACFLIRSGCDVNSPRQPGANGEGEEEARDGQTPLHLAASWGLEETVQCLLEFGANVNAQDAEGRTPIHVAISSQHGVIIQLLVSHPDIHLNVRDRQGLTPFACAMTFKNNKSAEAILKRESGAAEQVDNKGRNFLHVAVQNSDIESVLFLISVHANVNSRVQDASKLTPLHLAVQAGSEIIVRNLLLAGAKVNELTKHRQTALHLAAQQDLPTICSVLLENGVDFAAVDENGNNALHLAVMHGRLNNIRVLLTECTVDAEAFNLRGQSPLHILGQYGKENAAAIFDLFLECMPGYPLDKPDADGSTVLLLAYMKGNANLCRAIVRSGARLGVNNNQGVNIFNYQVATKQLLFRLLDMLSKEPPWCDGSYCYECTARFGVTTRKHHCRHCGRLLCHKCSTKEIPIIKFDLNKPVRVCNICFDVLTLGGVS
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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