ANKFY1 Protein, Human (sf9, His, StrepⅡ)

ANKFY1 is a proposed Rab5 effector that critically participates in endosomal dynamics by binding to PI(3)P, promoting homotypic early endosomal fusion and participating in heterotypic fusion. It plays a crucial role in macropinocytosis, is dependent on Rab5-GTP, and is essential for the correct localization of activated tyrosine kinase receptors such as PDGFRB. ANKFY1 Protein, Human (sf9, His, Strep) is the recombinant human-derived ANKFY1 protein, expressed by sf9 insect cells , with N-Strep, N-8*His labeled tag.

ANKFY1, a proposed effector of Rab5, plays a crucial role in endosomal dynamics by binding to phosphatidylinositol 3-phosphate (PI(3)P) and facilitating homotypic early endosome fusion. It also participates, albeit to a lesser extent, in the heterotypic fusion of clathrin-coated vesicles with early endosomes. ANKFY1 is intricately involved in macropinocytosis, a process dependent on Rab5-GTP. Additionally, ANKFY1 is essential for the correct endosomal localization and internalization of activated tyrosine kinase receptors, such as PDGFRB. Notably, it regulates the subcellular localization of the retromer complex in an EHD1-dependent manner, impacting endosome-to-Golgi transport and biosynthetic transport to late endosomes and lysosomes. Interactions with key proteins like RAB5A, RHOD, EHD1, and VPS26A underscore ANKFY1's role in coordinating various aspects of endosomal and retrograde transport processes.

Human

Sf9 insect cells

N-StrepⅡ;N-8*His

Q9P2R3 (A2-S1169)

51479

Full Length of Mature Protein

AEEEVAKLEKHLMLLRQEYVKLQKKLAETEKRCALLAAQANKESSSESFISRLLAIVADLYEQEQYSDLKIKVGDRHISAHKFVLAARSDSWSLANLSSTKELDLSDANPEVTMTMLRWIYTDELEFREDDVFLTELMKLANRFQLQLLRERCEKGVMSLVNVRNCIRFYQTAEELNASTLMNYCAEIIASHWDDLRKEDFSSMSAQLLYKMIKSKTEYPLHKAIKVEREDVVFLYLIEMDSQLPGKLNEADHNGDLALDLALSRRLESIATTLVSHKADVDMVDKSGWSLLHKGIQRGDLFAATFLIKNGAFVNAATLGAQETPLHLVALYSSKKHSADVMSEMAQIAEALLQAGANPNMQDSKGRTPLHVSIMAGNEYVFSQLLQCKQLDLELKDHEGSTALWLAVQHITVSSDQSVNPFEDVPVVNGTSFDENSFAARLIQRGSHTDAPDTATGNCLLQRAAGAGNEAAALFLATNGAHVNHRNKWGETPLHTACRHGLANLTAELLQQGANPNLQTEEALPLPKEAASLTSLADSVHLQTPLHMAIAYNHPDVVSVILEQKANALHATNNLQIIPDFSLKDSRDQTVLGLALWTGMHTIAAQLLGSGAAINDTMSDGQTLLHMAIQRQDSKSALFLLEHQADINVRTQDGETALQLAIRNQLPLVVDAICTRGADMSVPDEKGNPPLWLALANNLEDIASTLVRHGCDATCWGPGPGGCLQTLLHRAIDENNEPTACFLIRSGCDVNSPRQPGANGEGEEEARDGQTPLHLAASWGLEETVQCLLEFGANVNAQDAEGRTPIHVAISSQHGVIIQLLVSHPDIHLNVRDRQGLTPFACAMTFKNNKSAEAILKRESGAAEQVDNKGRNFLHVAVQNSDIESVLFLISVHANVNSRVQDASKLTPLHLAVQAGSEIIVRNLLLAGAKVNELTKHRQTALHLAAQQDLPTICSVLLENGVDFAAVDENGNNALHLAVMHGRLNNIRVLLTECTVDAEAFNLRGQSPLHILGQYGKENAAAIFDLFLECMPGYPLDKPDADGSTVLLLAYMKGNANLCRAIVRSGARLGVNNNQGVNIFNYQVATKQLLFRLLDMLSKEPPWCDGSYCYECTARFGVTTRKHHCRHCGRLLCHKCSTKEIPIIKFDLNKPVRVCNICFDVLTLGGVS

≥ 90%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.