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Apolipoprotein H/APOH Protein, Rat (HEK293, His)

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Based on 1 Customer Validation

Apolipoprotein H/APOH proteins have multiple functions and can bind to negatively charged substances such as heparin and phospholipids. Its diverse interactions highlight the adaptability that is critical for preventing activation of the intrinsic coagulation cascade by binding to phospholipids on the surface of damaged cells. Apolipoprotein H/APOH Protein, Rat (HEK293, His) is the recombinant rat-derived Apolipoprotein H/APOH protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Apolipoprotein H/APOH Protein, Rat (HEK293, His)
  • Experimental Validation Results for Apolipoprotein H/APOH Protein, Rat (HEK293, His)
  • Apolipoprotein H/APOH Protein, Rat (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Apolipoprotein H/APOH proteins have multiple functions and can bind to negatively charged substances such as heparin and phospholipids. Its diverse interactions highlight the adaptability that is critical for preventing activation of the intrinsic coagulation cascade by binding to phospholipids on the surface of damaged cells. Apolipoprotein H/APOH Protein, Rat (HEK293, His) is the recombinant rat-derived Apolipoprotein H/APOH protein, expressed by HEK293 , with C-His labeled tag.

Background

The Apolipoprotein H/APOH protein exhibits versatile functionality as it binds to various negatively charged substances, including heparin, phospholipids, and dextran sulfate. Its interactions with these substances suggest a broad spectrum of molecular engagements, highlighting its adaptability. Notably, Apolipoprotein H/APOH may play a crucial role in preventing the activation of the intrinsic blood coagulation cascade by specifically binding to phospholipids present on the surface of damaged cells. This functionality underscores its potential contribution to the regulation of coagulation processes and its importance in maintaining hemostatic balance in response to cellular damage.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Rat Apolipoprotein H Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human LDLR Protein. The ED50 for this effect is 0.7041 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. When Recombinant Rat Apolipoprotein H Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human LDLR Protein. The ED50 for this effect is 0.7041 μg/mL.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q5I0M1 (G20-C345)

  • Gene ID
    287774  [NCBI]
  • Molecular Construction
    • N-term
    • APOH (G20-C345)
      Accession # Q5I0M1
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    APOH; Anticardiolipin Cofactor; Prev. B2G1; Beta(2)GPI; Beta-2-Glycoprotein I; B2GPI; Beta-2-Glycoprotein 1; Apo-H; BG; Epididymis Secretory Sperm Binding Protein; Apolipoprotein H (Beta-2-Glycoprotein I); B2GP1; Activated Protein C-Binding Protein; Apoli

  • AA Sequence

    GRTCPKPDELPFAVVVPLKTFYDPGEQIVYSCKPGYVSRGGMRRFTCPLTGMWPINTLKCIPRVCPFAGILENGVVRYTTFEYPNTIGFACNPGYYLNGTSSSKCTEEGKWSPELPVCARITCPPPPIPKFAALKEYKTSVGNSSFYQDTVVFKCLPHFAMFGNDTVTCTAHGNWTQLPECREVKCPFPSRPDNGFVNYPAKPVLSYKDKAVFGCHETYKLDGPEEVECTKTGNWSALPSCKASCKLSVKKATVLYQGQRVKIQDQFKNGMMHGDKVHFYCKNKEKKCSYTEEAQCIDGTIEIPKCFKEHSSLAFWKTDASDVTPC

  • Molecular Weight

    Approximately 45-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. When Recombinant Rat Apolipoprotein H Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human LDLR Protein. The ED50 for this effect is 0.7041 μg/mL.

  • Apolipoprotein H/APOH Protein, Rat (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Apolipoprotein H/APOH Protein, Rat (HEK293, His)
  • Experimental Validation Results for Apolipoprotein H/APOH Protein, Rat (HEK293, His)
  • Apolipoprotein H/APOH Protein, Rat (HEK293, His) Product Image