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Apolipoprotein H/APOH Protein, Rat (HEK293, His)

Cat. No.: HY-P74410
SDS COA Handling Instructions

Apolipoprotein H/APOH proteins have multiple functions and can bind to negatively charged substances such as heparin and phospholipids. Its diverse interactions highlight the adaptability that is critical for preventing activation of the intrinsic coagulation cascade by binding to phospholipids on the surface of damaged cells. Apolipoprotein H/APOH Protein, Rat (HEK293, His) is the recombinant rat-derived Apolipoprotein H/APOH protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

Apolipoprotein H/APOH proteins have multiple functions and can bind to negatively charged substances such as heparin and phospholipids. Its diverse interactions highlight the adaptability that is critical for preventing activation of the intrinsic coagulation cascade by binding to phospholipids on the surface of damaged cells. Apolipoprotein H/APOH Protein, Rat (HEK293, His) is the recombinant rat-derived Apolipoprotein H/APOH protein, expressed by HEK293 , with C-His labeled tag.

Background

The Apolipoprotein H/APOH protein exhibits versatile functionality as it binds to various negatively charged substances, including heparin, phospholipids, and dextran sulfate. Its interactions with these substances suggest a broad spectrum of molecular engagements, highlighting its adaptability. Notably, Apolipoprotein H/APOH may play a crucial role in preventing the activation of the intrinsic blood coagulation cascade by specifically binding to phospholipids present on the surface of damaged cells. This functionality underscores its potential contribution to the regulation of coagulation processes and its importance in maintaining hemostatic balance in response to cellular damage.

Biological Activity

Measured by its binding ability in a functional ELISA. When Recombinant Rat Apolipoprotein H Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human LDLR Protein. The ED50 for this effect is 0.7041 μg/mL.

  • Measured by its binding ability in a functional ELISA. When Recombinant Rat Apolipoprotein H Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human LDLR Protein. The ED50 for this effect is 0.7041 μg/mL.
Species

Rat

Source

HEK293

Tag

C-6*His

Accession

Q5I0M1 (G20-C345)

Gene ID
Molecular Construction
N-term
APOH (G20-C345)
Accession # Q5I0M1
His
C-term
Synonyms
Apolipoprotein H; ApoH; B2G1; B2GP1
AA Sequence

GRTCPKPDELPFAVVVPLKTFYDPGEQIVYSCKPGYVSRGGMRRFTCPLTGMWPINTLKCIPRVCPFAGILENGVVRYTTFEYPNTIGFACNPGYYLNGTSSSKCTEEGKWSPELPVCARITCPPPPIPKFAALKEYKTSVGNSSFYQDTVVFKCLPHFAMFGNDTVTCTAHGNWTQLPECREVKCPFPSRPDNGFVNYPAKPVLSYKDKAVFGCHETYKLDGPEEVECTKTGNWSALPSCKASCKLSVKKATVLYQGQRVKIQDQFKNGMMHGDKVHFYCKNKEKKCSYTEEAQCIDGTIEIPKCFKEHSSLAFWKTDASDVTPC

Molecular Weight

Approximately 45-70 kDa due to the glycosylation.

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Apolipoprotein H/APOH Protein, Rat (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Apolipoprotein H/APOH Protein, Rat (HEK293, His)
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HY-P74410
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