Apolipoprotein H/APOH Protein, Rat (HEK293, His)

Apolipoprotein H/APOH proteins have multiple functions and can bind to negatively charged substances such as heparin and phospholipids. Its diverse interactions highlight the adaptability that is critical for preventing activation of the intrinsic coagulation cascade by binding to phospholipids on the surface of damaged cells. Apolipoprotein H/APOH Protein, Rat (HEK293, His) is the recombinant rat-derived Apolipoprotein H/APOH protein, expressed by HEK293 , with C-His labeled tag. The total length of Apolipoprotein H/APOH Protein, Rat (HEK293, His) is 345 a.a., with molecular weight of 45-70 kDa.

The Apolipoprotein H/APOH protein exhibits versatile functionality as it binds to various negatively charged substances, including heparin, phospholipids, and dextran sulfate. Its interactions with these substances suggest a broad spectrum of molecular engagements, highlighting its adaptability. Notably, Apolipoprotein H/APOH may play a crucial role in preventing the activation of the intrinsic blood coagulation cascade by specifically binding to phospholipids present on the surface of damaged cells. This functionality underscores its potential contribution to the regulation of coagulation processes and its importance in maintaining hemostatic balance in response to cellular damage.

Measured by its binding ability in a functional ELISA. When Recombinant Rat Apolipoprotein H Protein is immobilized at 2 µg/mL (100 µL/well) can bind Biotinylated Human LDLR Protein. The ED₅₀ for this effect is 0.7041 μg/mL.

Rat

HEK293

C-6*His

Q5I0M1 (G20-C345)

287774  [NCBI]

GRTCPKPDELPFAVVVPLKTFYDPGEQIVYSCKPGYVSRGGMRRFTCPLTGMWPINTLKCIPRVCPFAGILENGVVRYTTFEYPNTIGFACNPGYYLNGTSSSKCTEEGKWSPELPVCARITCPPPPIPKFAALKEYKTSVGNSSFYQDTVVFKCLPHFAMFGNDTVTCTAHGNWTQLPECREVKCPFPSRPDNGFVNYPAKPVLSYKDKAVFGCHETYKLDGPEEVECTKTGNWSALPSCKASCKLSVKKATVLYQGQRVKIQDQFKNGMMHGDKVHFYCKNKEKKCSYTEEAQCIDGTIEIPKCFKEHSSLAFWKTDASDVTPC

Approximately 45-70 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.