ATP2A1 Protein, Human (Cell-Free, His)
ATP2A1 is critical for striated muscle function, acting as the primary Ca(2+) ATPase to efficiently reuptake cytosolic Ca(2+) into the sarcoplasmic reticulum. Through ATP hydrolysis, ATP2A1 transfers calcium from the cytoplasm to the sarcoplasmic reticulum lumen, which is critical for precise control of calcium levels, regulation of isolation, and control of muscle excitation and contraction dynamics. ATP2A1 Protein, Human (Cell-Free, His) is the recombinant human-derived ATP2A1 protein, expressed by E. coli Cell-free, with N-10*His labeled tag.
- Species: Human
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ATP2A1 is critical for striated muscle function, acting as the primary Ca(2+) ATPase to efficiently reuptake cytosolic Ca(2+) into the sarcoplasmic reticulum. Through ATP hydrolysis, ATP2A1 transfers calcium from the cytoplasm to the sarcoplasmic reticulum lumen, which is critical for precise control of calcium levels, regulation of isolation, and control of muscle excitation and contraction dynamics. ATP2A1 Protein, Human (Cell-Free, His) is the recombinant human-derived ATP2A1 protein, expressed by E. coli Cell-free, with N-10*His labeled tag.
Background
ATP2A1, a pivotal player in striated muscle function, serves as the primary Ca(2+) ATPase responsible for the efficient reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Operating through the hydrolysis of ATP, ATP2A1 facilitates the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, a process crucial for the regulation of calcium sequestration and the intricate dynamics of muscular excitation and contraction. This intricate molecular mechanism highlights the indispensable role of ATP2A1 in orchestrating the precise control of calcium levels within muscle cells, ultimately contributing to the overall performance and functionality of striated muscles.
Technical Parameters
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Species Human
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Source E. coli Cell-free
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Tag N-10*His
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Accession
O14983 (E10-E450)
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Gene ID784
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Molecular Construction
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N-term
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10*His
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ATP2A1 (E10-E450)
Accession # O14983 -
C-term
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Protein Length
Partial
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Synonyms
ATP2A1; Calcium Pump 1; Prev. ATP2A; Endoplasmic Reticulum Class 1/2 Ca(2+) ATPase; SERCA1; SR Ca(2+)-ATPase 1; Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 1; Calcium-Transporting ATPase Sarcoplasmic Reticulum Type, Fast Twitch Skeletal Muscle Isofo
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AA Sequence
EECLAYFGVSETTGLTPDQVKRNLEKYGLNELPAEEGKTLWELVIEQFEDLLVRILLLAACISFVLAWFEEGEETITAFVEPFVILLILIANAIVGVWQERNAENAIEALKEYEPEMGKVYRADRKSVQRIKARDIVPGDIVEVAVGDKVPADIRILAIKSTTLRVDQSILTGESVSVIKHTEPVPDPRAVNQDKKNMLFSGTNIAAGKALGIVATTGVGTEIGKIRDQMAATEQDKTPLQQKLDEFGEQLSKVISLICVAVWLINIGHFNDPVHGGSWFRGAIYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMAKKNAIVRSLPSVETLGCTSVICSDKTGTLTTNQMSVCKMFIIDKVDGDICLLNEFSITGSTYAPEGEVLKNDKPVRPGQYDGLVELATICALCNDSSLDFNEAKGVYEKVGEATETALTTLVE
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Predicted Molecular Mass
49.5 kDa
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Molecular Weight
Approximately 50 kDa, based on SDS-PAGE under reducing conditions.
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Structure/Form
Monomer
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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