ABCC1 Protein, Bovine (HEK293, GFP, StrepⅡ, His)
The ABCC1 protein plays a key role in cellular physiology, mediating the ATP-dependent export of a variety of substrates, including drugs and organic anions. Notably, it confers resistance to anticancer drugs, actively reducing their intracellular accumulation. ABCC1 Protein, Bovine (HEK293, GFP, Strep, His) is the recombinant bovine-derived ABCC1 protein, expressed by HEK293 , with C-10*His, C-GFP, C-StrepII labeled tag.
- Species: Bovine
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The ABCC1 protein plays a key role in cellular physiology, mediating the ATP-dependent export of a variety of substrates, including drugs and organic anions. Notably, it confers resistance to anticancer drugs, actively reducing their intracellular accumulation. ABCC1 Protein, Bovine (HEK293, GFP, Strep, His) is the recombinant bovine-derived ABCC1 protein, expressed by HEK293 , with C-10*His, C-GFP, C-StrepII labeled tag.
Background
ABCC1 Protein assumes a pivotal role in cellular physiology by mediating the export of organic anions and diverse drugs from the cytoplasm. This multifunctional transporter facilitates the ATP-dependent transport of various substrates, including glutathione and its conjugates, leukotriene C4, estradiol-17-beta-o-glucuronide, methotrexate, and antiviral drugs. Remarkably, bABCC1 confers resistance to anticancer drugs by actively reducing the intracellular accumulation of these agents through ATP- and GSH-dependent drug export mechanisms. Despite its relatively low efficiency in ATP hydrolysis, bABCC1 plays a crucial role in exporting sphingosine 1-phosphate from mast cells independently of degranulation, and also participates in the inflammatory response by facilitating the export of leukotriene C4 from cells involved in leukotriene C4 synthesis. Additionally, bABCC1 mediates ATP-dependent, GSH-independent export of cyclic GMP-AMP (cGAMP), thus acting as a negative regulator of the cGAS-STING pathway by limiting intracellular cGAMP concentrations. The broad spectrum of substrates and its impact on drug resistance underscore the importance of bABCC1 in cellular homeostasis and therapeutic response, warranting further investigation into its precise molecular mechanisms and therapeutic implications.
Technical Parameters
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Species Bovine
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Source HEK293
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Tag C-StrepⅡ;C-10*His;C-GFP
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Accession
Q8HXQ5 (M1-V1530)
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Gene ID281588
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Molecular Construction
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N-term
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ABCC1 (M1-V1530)
Accession # Q8HXQ5 -
GFP-StrepⅡ-10*His
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C-term
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Protein Length
Full Length
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Synonyms
ABCC1; ATP-Binding Cassette Sub-Family C Member 1; Prev. MRP1; LTC4 Transporter; Prev. MRP; ATP-Binding Cassette Transporter Variant ABCC1delta-Ex25; Glutathione-S-Conjugate-Translocating ATPase ABCC1; ATP-Binding Cassette Transporter Variant ABCC1delta-E
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AA Sequence
MALRDFCSVDGSDLFWEWNVTWNTSNPDFTKCFQNTVLVWVPCSYLWVCFPFYFLYLSHHDRGYIQMTHLNKAKTALGFLLWIVCWADLFYSFWERSMGKLLAPVFLVSPTLLGITMLLATFLIQIERRRGVQSSGIMLTFWLIALLCALAILRSKIMTALKEDARVDVFRDVTFYIYFSLVLIQLVLSCFSDRSPLFSETINDPNPCPESSASFLSRITFWWITGMMVQGYRQPLESTDLWSLNKEDTSEQVVPVLVKNWKKECAKSRKQPVKIVYSSKDPAKPKGSSKVDVNEEAEALIVKCPQKERDPSLFKVLYKTFGPYFLMSFLFKAVHDLMMFAGPEILKLLINFVNDKKAPEWQGYFYTALLFISACLQTLVLHQYFHICFVSGMRIKTAVIGAVYRKALVITNAARKSSTVGEIVNLMSVDAQRFMDLATYINMIWSAPLQVILALYLLWLNLGPSVLAGVAVMVLMVPLNAVMAMKTKTYQVAHMKSKDNRIKLMNEILNGIKVLKLYAWELAFKDKVLAIRQEELKVLKKSAYLAAVGTFTWVCTPFLVALSTFAVYVTVDENNILDAQKAFVSLALFNILRFPLNILPMVISSIVQASVSLKRLRVFLSHEDLDPDSIQRRPIKDAGATNSITVKNATFTWARNDPPTLHGITFSVPEGSLVAVVGQVGCGKSSLLSALLAEMDKVEGHVTVKGSVAYVPQQAWIQNISLRENILFGRQLQERYYKAVVEACALLPDLEILPSGDRTEIGEKGVNLSGGQKQRVSLARAVYCDSDVYLLDDPLSAVDAHVGKHIFENVIGPKGLLKNKTRLLVTHAISYLPQMDVIIVMSGGKISEMGSYQELLARDGAFAEFLRTYASAEQEQGQPEDGLAGVGGPGKEVKQMENGMLVTDTAGKQMQRQLSSSSSYSRDVSQHHTSTAELRKPGPTEETWKLVEADKAQTGQVKLSVYWDYMKAIGLFISFLSIFLFLCNHVASLVSNYWLSLWTDDPIVNGTQEHTQVRLSVYGALGISQGITVFGYSMAVSIGGIFASRRLHLDLLHNVLRSPISFFERTPSGNLVNRFSKELDTVDSMIPQVIKMFMGSLFNVIGACIIILLATPMAAVIIPPLGLIYFFVQRFYVASSRQLKRLESVSRSPVYSHFNETLLGVSVIRAFEEQERFIRQSDLKVDENQKAYYPSIVANRWLAVRLECVGNCIVLFASLFAVISRHSLSAGLVGLSVSYSLQVTTYLNWLVRMSSEMETNIVAVERLKEYSETEKEAPWQIQDMAPPKDWPQVGRVEFRDYGLRYREDLDLVLKHINVTIDGGEKVGIVGRTGAGKSSLTLGLFRIKESAEGEIIIDDINIAKIGLHDLRFKITIIPQDPVLFSGSLRMNLDPFSQYSDEEVWTSLELAHLKGFVSALPDKLNHECAEGGENLSVGQRQLVCLARALLRKTKILVLDEATAAVDLETDDLIQSTIRTQFDDCTVLTIAHRLNTIMDYTRVIVLDKGEIQEWGSPSDLLQQRGLFYSMAKDSGLV
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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