2. Recombinant Proteins
  3. Enzymes & Regulators Kinases
  4. Transferases (EC 2) Atypical Kinases
  5. PDHK
  6. BCKDK
  7. BCKDK Protein, Human (His, Strep)

BCKDK Protein, Human (His, Strep) is the recombinant human-derived BCKDK, expressed by E. coli, with Strep, His labeled tag.

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Descripciòn

BCKDK Protein, Human (His, Strep) is the recombinant human-derived BCKDK, expressed by E. coli, with Strep, His labeled tag.
Background

BCKDK is a serine/threonine-protein kinase that functions as a metabolic regulator by forming a kinase-phosphatase pair with PPM1K. It coordinates branched-chain amino acid (BCAA) catabolism with glucose and lipid metabolism through phosphorylation of two distinct targets: 1) the mitochondrial BCKDHA subunit of BCKDH complex (inactivating it via Ser-337 phosphorylation to disrupt substrate channeling), and 2) cytosolic ACLY (phosphorylating Ser-455 during feeding transitions to promote lipogenesis/gluconeogenesis via MLXIPL/ChREBP-mediated transcriptional regulation). Recognizes phosphosites with SxxE/D motif.
Species

Human
Source

E. coli
Tag

Strep;His
Accession

O14874-1 (S31-I412)
Gene ID

10295
Protein Length

Full Length of Mature Protein
Synonyms
Branched-chain alpha-ketoacid dehydrogenase kinase; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial; 2.7.11.4; BCKDK; Homo sapiens; Human; Kinase; Transferase; 2.7.11.1
AA Sequence

STSATDTHHVEMARERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDGREESFRI

Predicted Molecular Mass
46.5 kDa
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentación
Instrucciones de manejo (2659 KB)

BCKDK Protein, Human (His, Strep) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Nombre del producto:
BCKDK Protein, Human (His, Strep)
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