2. 組換えタンパク質
  3. Enzymes & Regulators Kinases
  4. Transferases (EC 2) Atypical Kinases
  5. PDHK
  6. BCKDK
  7. BCKDK Protein, Human (sf9, GST)

BCKDK Protein, Human (sf9, GST) is the recombinant human-derived BCKDK, expressed by Sf9 insect cells , with GST labeled tag.

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Surface Plasmon Resonance (SPR) アッセイサ-ビス   Protein Expression Service

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製品説明

BCKDK Protein, Human (sf9, GST) is the recombinant human-derived BCKDK, expressed by Sf9 insect cells , with GST labeled tag.
Background

BCKDK is a serine/threonine-protein kinase that functions as a metabolic regulator by forming a kinase-phosphatase pair with PPM1K. It coordinates branched-chain amino acid (BCAA) catabolism with glucose and lipid metabolism through phosphorylation of two distinct targets: 1) the mitochondrial BCKDHA subunit of BCKDH complex (inactivating it via Ser-337 phosphorylation to disrupt substrate channeling), and 2) cytosolic ACLY (phosphorylating Ser-455 during feeding transitions to promote lipogenesis/gluconeogenesis via MLXIPL/ChREBP-mediated transcriptional regulation). Recognizes phosphosites with SxxE/D motif.
Species

Human
Source

Sf9 insect cells
Tag

GST
Accession

O14874-1 (R30-I412)
Gene ID

10295
Protein Length

Full Length of Mature Protein
Synonyms
Branched-chain alpha-ketoacid dehydrogenase kinase; BCKDH kinase; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase; mitochondrial
AA Sequence

RSTSATDTHHVEMARERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDGREESFRI

Predicted Molecular Mass
69 kDa
純度

≥ 95%, as determined by reducing SDS-PAGE.
ドキュメンテーション
取扱説明書 (2659 KB)

BCKDK Protein, Human (sf9, GST) 関連分類

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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製品名:
BCKDK Protein, Human (sf9, GST)
製品番号:
HY-P702948
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