BCKDK Protein, Human (sf9, GST)
BCKDK Protein, Human (sf9, GST) is the recombinant human-derived BCKDK, expressed by Sf9 insect cells , with GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
BCKDK Protein, Human (sf9, GST) is the recombinant human-derived BCKDK, expressed by Sf9 insect cells , with GST labeled tag.
Background
BCKDK is a serine/threonine-protein kinase that functions as a metabolic regulator by forming a kinase-phosphatase pair with PPM1K. It coordinates branched-chain amino acid (BCAA) catabolism with glucose and lipid metabolism through phosphorylation of two distinct targets: 1) the mitochondrial BCKDHA subunit of BCKDH complex (inactivating it via Ser-337 phosphorylation to disrupt substrate channeling), and 2) cytosolic ACLY (phosphorylating Ser-455 during feeding transitions to promote lipogenesis/gluconeogenesis via MLXIPL/ChREBP-mediated transcriptional regulation). Recognizes phosphosites with SxxE/D motif.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag GST
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Accession
O14874-1 (R30-I412)
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Gene ID10295
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Protein Length
Full Length of Isoform-1
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Synonyms
BCKDK; BDK; Branched Chain Keto Acid Dehydrogenase Kinase; Branched Chain Alpha-Ketoacid Dehydrogenase Kinase; [3-Methyl-2-Oxobutanoate Dehydrogenase [Lipoamide]] Kinase, Mitochondrial; Branched Chain Ketoacid Dehydrogenase Kinase; Branched-Chain Alpha-Ke
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AA Sequence
RSTSATDTHHVEMARERSKTVTSFYNQSAIDAAAEKPSVRLTPTMMLYAGRSQDGSHLLKSARYLQQELPVRIAHRIKGFRCLPFIIGCNPTILHVHELYIRAFQKLTDFPPIKDQADEAQYCQLVRQLLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHHLALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGHVAARFPFIPMPLDYILPELLKNAMRATMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHFTTAEASTQDPRISPLFGHLDMHSGAQSGPMHGFGFGLPTSRAYAEYLGGSLQLQSLQGIGTDVYLRLRHIDGREESFRI
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Molecular Weight
Approximately 69 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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