Alpha 1-Microglobulin Protein, Rat (HEK293, His)

Alpha 1-microglobulin (called bikunin) plays multiple roles: maintaining extracellular matrix and cell membrane structure, acting as an antioxidant at the blood-placenta interface, maintaining mitochondrial redox homeostasis, and protecting renal tubular cells from heme induction of oxidative damage. It acts as a serine protease inhibitor and contributes to extracellular space remodeling, cell adhesion and cumulus expansion during ovulation. Alpha 1-Microglobulin Protein, Rat (HEK293, His) is the recombinant rat-derived Alpha 1-Microglobulin protein, expressed by HEK293 , with C-His labeled tag. The total length of Alpha 1-Microglobulin Protein, Rat (HEK293, His) is 202 a.a., with molecular weight of ~25-30 KDa.

Bikunin is involved in maintaining the structure and function of the extracellular matrix and cell membranes. It acts as an antioxidant by reducing oxidized collagen fibers and preventing oxidative damage at the blood-placenta interface. It also helps maintain mitochondrial redox homeostasis by scavenging free radicals and preserving ATP synthesis. Bikunin protects renal tubule epithelial cells from heme-induced oxidative damage to mitochondria. It has a chaperone role in facilitating the correct folding of bikunin itself in the endoplasmic reticulum. Bikunin is a serine protease inhibitor and plays a role in extracellular space remodeling and cell adhesion. It inhibits various proteases involved in inflammation and cytotoxic responses. Bikunin is also involved in the transfer of heavy chains from the I-alpha-I complex to hyaluronan, allowing for the remodeling of extracellular matrix proteoglycan structures. Additionally, it plays a role in cumulus oophorus expansion during ovulation and inhibits calcium oxalate crystallization.

Rat

HEK293

C-6*His

Q64240 (D20-A202)

25377  [NCBI]

DNVPTLPDIQVQENFNEARIYGKWFNLAVGSTCPWLRRIKNKMSVSTLVLQEGATEAEISVTSTQWRKGVCEEISGVYQKTDIDGKFLYHKSKWNATLESYVVHTNYDEYAIFLTKKFSHRHGPTITAKLYGREPQLRDSLLQEFREVALSVGIPENSIVFMADRGECVPGDREVESTSFARA

Approximately 25-30 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.