2. Recombinant Proteins
  3. Enzymes & Regulators Kinases
  4. Transferases (EC 2) Pseudokinases TKL
  5. RAF
  6. BRAF Protein, Human (Active, sf9, His-GST)

BRAF Protein, Human (Active, sf9, His-GST) is the recombinant human-derived BRAF, expressed by Sf9 insect cells .

For research use only. We do not sell to patients.

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Description

BRAF Protein, Human (Active, sf9, His-GST) is the recombinant human-derived BRAF, expressed by Sf9 insect cells .
Background

BRAF is a protein kinase involved in transducing mitogenic signals from the cell membrane to the nucleus (Probable). It phosphorylates MAP2K1, activating the MAP kinase signal transduction pathway (by similar: PubMed:21441910, PubMed:29433126), and can phosphorylate PFKFB2 (by similar: PubMed:36402789). Additionally, it may play a role in the postsynaptic responses of hippocampal neurons (by similar: PubMed:1508179).
Species

Human
Source

Sf9 insect cells
Tag

N-His;N-GST
Accession

P15056 (D381-H766)
Gene ID

673
Molecular Construction
N-term
His-GST
BRAF (D381-H766)
Accession # P15056
C-term
Protein Length

Partial
Synonyms
BRAF1; RAFB; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1
Predicted Molecular Mass
70.8 KDa
Molecular Weight

Approximately 61.3 kDa
Purity

≥ 80%, as determined by reducing SDS-PAGE.
Documentation
Handling Instructions (2659 KB)

BRAF Protein, Human (Active, sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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