C-Reactive Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
C-reactive protein (CRP) has cholesterol-binding, complement C1q complex-binding, and low-density lipoprotein particle-binding activities, and plays a role in complement activation, gene expression regulation, and superoxide anion generation. It is located in filopodia and growth cones and is expressed in embryos and liver. C-Reactive Protein, Mouse (HEK293, His) is the recombinant mouse-derived C-Reactive protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
C-reactive protein (CRP) has cholesterol-binding, complement C1q complex-binding, and low-density lipoprotein particle-binding activities, and plays a role in complement activation, gene expression regulation, and superoxide anion generation. It is located in filopodia and growth cones and is expressed in embryos and liver. C-Reactive Protein, Mouse (HEK293, His) is the recombinant mouse-derived C-Reactive protein, expressed by HEK293 , with C-His labeled tag.
Background
The C-reactive protein (CRP), predicted to possess several functions such as cholesterol binding activity, complement component C1q complex binding activity, and low-density lipoprotein particle binding activity, is anticipated to play a role in diverse processes, including complement activation via the classical pathway, regulation of gene expression, and the regulation of superoxide anion generation. It is predicted to act upstream of vasoconstriction and is expected to be located in filopodia and growth cones, with activity in the extracellular space. This protein is expressed in embryos and the liver. The human ortholog of CRP is implicated in various diseases, including Kawasaki disease, autoimmune diseases, macular degeneration, middle cerebral artery infarction, and nephritis. Notably, CRP exhibits biased expression in tissues such as the liver during embryonic and adult stages.
Verified Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized C-Reactive at 1 μg/mL(100 μL/well) can bind biotinylated Fc gamma RIIA. The ED50 for this effect is 0.8967 μg/mL.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - ELISA
Bioactivity - ELISA
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-His
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Accession
P14847/NP_031794.3 (H20-S225)
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Molecular Construction
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N-term
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C-Reactive (H20-S225)
Accession # P14847/NP_031794.3 -
His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
CRP; C-Reactive Protein, Pentraxin-Related; C-Reactive Protein; Pentraxin 1; PTX1
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AA Sequence
HEDMFKKAFVFPKESDTSYVSLEAESKKPLNTFTVCLHFYTALSTVRSFSVFSYATKKNSNDILIFWNKDKQYTFGVGGAEVRFMVSEIPEAPTHICASWESATGIVEFWIDGKPKVRKSLHKGYTVGPDASIILGQEQDSYGGDFDAKQSLVGDIGDVNMWDFVLSPEQISTVYVGGTLSPNVLNWRALNYKAQGDVFIKPQLWS
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Molecular Weight
Approximately 26-30 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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