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C-Reactive Protein, Rat (HEK293, His)

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Reactive protein (CRP) performs multiple functions of host defense, including promoting agglutination, bacterial capsule swelling, phagocytosis, and complement fixation.These activities are promoted by its calcium-dependent binding to phosphocholine, suggesting its role in orchestrating immune responses against pathogens.C-Reactive Protein, Rat (HEK293, His) is the recombinant rat-derived C-Reactive protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Reactive protein (CRP) performs multiple functions of host defense, including promoting agglutination, bacterial capsule swelling, phagocytosis, and complement fixation.These activities are promoted by its calcium-dependent binding to phosphocholine, suggesting its role in orchestrating immune responses against pathogens.C-Reactive Protein, Rat (HEK293, His) is the recombinant rat-derived C-Reactive protein, expressed by HEK293 , with C-His labeled tag.

Background

C-Reactive Protein (CRP) plays a multifaceted role in host defense, demonstrating several functions essential for immune responses. CRP promotes agglutination, bacterial capsular swelling, phagocytosis, and complement fixation by binding to phosphorylcholine in a calcium-dependent manner. Additionally, CRP exhibits the ability to interact with DNA and histones, suggesting a potential role in scavenging nuclear material released from damaged circulating cells. These diverse functions underscore CRP's involvement in various aspects of the immune system, contributing to both innate and adaptive immune responses, and highlight its significance as a key player in host defense mechanisms.

Verified Bioactivity

Immobilized Recombinant C-Reactive Protein/CRP at 1 μg/ml (100 μL/well) can bind Biotinylated Recombinant Human Fc gamma RIIA/CD32a Protein. The ED50 for this effect is 1.821 μg/mL.

Technical Parameters

  • Species Rat
  • Source HEK293
  • Tag C-His
  • Accession

    P48199 (H20-S230)

  • Gene ID
    25419  [NCBI]
  • Molecular Construction
    • N-term
    • C-Reactive (H20-S230)
      Accession # P48199
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CRP; C-Reactive Protein, Pentraxin-Related; C-Reactive Protein; Pentraxin 1; PTX1

  • AA Sequence

    HEDMSKQAFVFPGVSATAYVSLEAESKKPLEAFTVCLYAHADVSRSFSIFSYATKTSFNEILLFWTRGQGFSIAVGGPEILFSASEIPEVPTHICATWESATGIVELWLDGKPRVRKSLQKGYIVGTNASIILGQEQDSYGGGFDANQSLVGDIGDVNMWDFVLSPEQINAVYVGRVFSPNVLNWRALKYETHGDVFIKPQLWPLTDCCES

  • Molecular Weight

    Approximately 30 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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