C-Reactive Protein, Rat (HEK293, His)

Reactive protein (CRP) performs multiple functions of host defense, including promoting agglutination, bacterial capsule swelling, phagocytosis, and complement fixation. These activities are promoted by its calcium-dependent binding to phosphocholine, suggesting its role in orchestrating immune responses against pathogens. C-Reactive Protein, Rat (HEK293, His) is the recombinant rat-derived C-Reactive protein, expressed by HEK293 , with C-His labeled tag. The total length of C-Reactive Protein, Rat (HEK293, His) is 211 a.a., with molecular weight of ~30 kDa.

C-Reactive Protein (CRP) plays a multifaceted role in host defense, demonstrating several functions essential for immune responses. CRP promotes agglutination, bacterial capsular swelling, phagocytosis, and complement fixation by binding to phosphorylcholine in a calcium-dependent manner. Additionally, CRP exhibits the ability to interact with DNA and histones, suggesting a potential role in scavenging nuclear material released from damaged circulating cells. These diverse functions underscore CRP's involvement in various aspects of the immune system, contributing to both innate and adaptive immune responses, and highlight its significance as a key player in host defense mechanisms.

Immobilized Recombinant C-Reactive Protein/CRP at 1 μg/ml (100 μL/well) can bind Biotinylated Recombinant Human Fc gamma RIIA/CD32a Protein. The ED₅₀ for this effect is 1.821 μg/mL.

Rat

HEK293

C-His

P48199 (H20-S230)

25419  [NCBI]

HEDMSKQAFVFPGVSATAYVSLEAESKKPLEAFTVCLYAHADVSRSFSIFSYATKTSFNEILLFWTRGQGFSIAVGGPEILFSASEIPEVPTHICATWESATGIVELWLDGKPRVRKSLQKGYIVGTNASIILGQEQDSYGGGFDANQSLVGDIGDVNMWDFVLSPEQINAVYVGRVFSPNVLNWRALKYETHGDVFIKPQLWPLTDCCES

Approximately 30 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.