Carboxylesterase 5 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
Carboxylesterase 5 Protein, Mouse (HEK293, His) is a mouse Carboxylesterase 5 protein with a His-flag. Carboxylesterase 5 is encoded byCarboxylesterase 5 (CES5) and can catalyses hydrolytic and transesterification reactions.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Carboxylesterase 5 Protein, Mouse (HEK293, His) is a mouse Carboxylesterase 5 protein with a His-flag. Carboxylesterase 5 is encoded byCarboxylesterase 5 (CES5) and can catalyses hydrolytic and transesterification reactions[1][2].
Background
Carbonic Anhydrase 11 belongs to the carbonic anhydrase (CA) family and may be involved in cell proliferation and cellular transformation. CAs are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide and thus participate in a variety of biological and physical processes[1]. Carboxylesterase 5 (CES5) is one of at least five mammalian CES gene families encoding enzymes of broad substrate specificity and catalysing hydrolytic and transesterification reactions[2].
Verified Bioactivity
Measured by its ability to hydrolyze 1mM 4-Nitrophenyl acetate that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 10573.649- 37513.038 pmol/min/μg.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-6*His
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Accession
Q8BK48 (Q27-R555)
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Gene ID234673 [NCBI]
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Molecular Construction
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N-term
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CES5 (Q27-R555)
Accession # Q8BK48 -
6*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
Ces5; Ces2e; carboxylesterase 2E; EC:3.1.1.-; Carboxylic ester hydrolase; EC:3.1.1.88; Pyrethroid hydrolase Ces2e
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AA Sequence
QDSASPIRNTHTGQVRGSLVHVKDTDIAVHTFLGIPFAKPPVGPLRFAPPEAPEPWSGVRDGTSHPNMCLQNDNLMGSEDLKMMNLILPPISMSEDCLYLNIYVPAHAHEGSNLPVMVWIHGGALTVGMASMYDGSMLAATEDVVVVAIQYRLGVLGFFSTGDQHAKGNWGYLDQVAALRWVQQNIVHFGGNPDRVTIFGESAGGTSVSSHVVSPMSQGLFHGAIMESGVAVLPDLISSSSEMVHRIVANLSGCAAVNSETLMCCLRGKNEAEMLAINKVFKIIPGVVDGEFLPKHPQELMASKDFHPVPSIIGINNDEYGWILPTIMDPAQKIEEITRKTLPAVLKSTALKMMLPPECGDLLMEEYMGDTEDPETLQAQFREMKGDFMFVIPALQVAHFQRSHAPVYFYEFQHRPSFFKDFRPPYVKADHGDEIFLVFGYQFGNIKLPYTEEEEQLSRRIMKYWANFARHGNPNSEGLPYWPVMDHDEQYLQLDIQPSVGRALKARRLQFWTKTLPQKIQELKGSQER
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Predicted Molecular Mass
60.2 kDa
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Molecular Weight
Approximately 58-65 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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