Carboxypeptidase E/CPE Protein, Human (428a.a, HEK293, His)
Based on 1 Customer Validation
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Background
Carboxypeptidase E (CPE), which cleaves C-terminal amino acid residues and is involved in neuropeptide processing, is itself subject to intracellular processing Carboxypeptidase E (CPE) is an enkephalin-convertase that cleaved a C-terminal basic residue from enkephalin precursors to generate enkephalin[1].CPE is a glycoprotein, which is synthesized as a larger inactive precursor. It is exclusively synthesized by membrane-bound polyribosomes in the brain[1][2]. There exists two forms of CPE, soluble and membrane-bound in most tissues with CPE activity. These two forms differ slightly in molecular weight, but have identical enzymatic properties. Both forms arise from the same precursor, which is encoded by a single gene. This gene is a member of a carboxypeptidase gene family that includes CPA and CPB[2].
Verified Bioactivity
Measured by its ability to cleave a peptide substrate, benzoyl-AR-OH. The product, Arg, reacted with orthophthaldialdehyde (OPA) to form a fluorescent molecule. The specific activity is >12,000 pmoles/min/μg.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
P16870-1/NP_001864.1 (E26-S453)
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Gene ID1363
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Molecular Construction
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N-term
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CPE (E26-S453)
Accession # P16870-1/NP_001864.1 -
His
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C-term
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Protein Length
Partial
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Synonyms
CPE; Insulin Granule-Associated Carboxypeptidase; Carboxypeptidase E; CPH; Enkephalin Convertase; Epididymis Secretory Sperm Binding Protein; Carboxypeptidase H; Carbocypeptidase E; Prohormone-Processing Carboxypeptidase; IDDHH; Cobalt-Stimulated Chromaff
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AA Sequence
EAQEPGAPAAGMRRRRRLQQEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNMKKIVDQNTKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGSAHEYSSSPDDAIFQSLARAYSSFNPAMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKTYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVEGIDHDVTSAKDGDYWRLLIPGNYKLTASAPGYLAITKKVAVPYSPAAGVDFELESFS
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Predicted Molecular Mass
49.4 kDa
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Molecular Weight
Approximately 53 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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