Carboxypeptidase E/CPE Protein, Human (HEK293 His)

Carboxypeptidase E Protein, Human (HEK293 His) is an approximately 57.0 kDa Carboxypeptidase E protein with His-flag, expressed in HEK293-cell system.

Carboxypeptidase E (CPE), which cleaves C-terminal amino acid residues and is involved in neuropeptide processing, is itself subject to intracellular processing Carboxypeptidase E (CPE) is an enkephalin-convertase that cleaved a C-terminal basic residue from enkephalin precursors to generate enkephalin^[1].
CPE is a glycoprotein, which is synthesized as a larger inactive precursor. It is exclusively synthesized by membrane-bound polyribosomes in the brain^[1][2].
There exists two forms of CPE, soluble and membrane-bound in most tissues with CPE activity. These two forms differ slightly in molecular weight, but have identical enzymatic properties. Both forms arise from the same precursor, which is encoded by a single gene. This gene is a member of a carboxypeptidase gene family that includes CPA and CPB^[2].

Human

HEK293

C-6*His

P16870-1 (R42-S453)

1363  [NCBI]

Partial

RLQQEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNMKKIVDQNTKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGSAHEYSSSPDDAIFQSLARAYSSFNPAMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKTYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVEGIDHDVTSAKDGDYWRLLIPGNYKLTASAPGYLAITKKVAVPYSPAAGVDFELESFS

Approximately 57.0 kDa

≥ 95%, as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.

• [1]. E Manser, et al. Human carboxypeptidase E. Isolation and characterization of the cDNA, sequence conservation, expression and processing in vitroBiochem J. 1990 Apr 15;267(2):517-25.  [Content Brief]

  [2]. Lloyd D Fricker, et al.Carboxypeptidase E and the Identification of Novel Neuropeptides as Potential Therapeutic Targets. Adv Pharmacol  [Content Brief]