Carboxypeptidase E/CPE Protein, Human (HEK293 His)
Based on 1 Customer Validation
Carboxypeptidase E Protein, Human (HEK293 His) is an approximately 57.0 kDa Carboxypeptidase E protein with His-flag, expressed in HEK293-cell system.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Carboxypeptidase E Protein, Human (HEK293 His) is an approximately 57.0 kDa Carboxypeptidase E protein with His-flag, expressed in HEK293-cell system.
Background
Carboxypeptidase E (CPE), which cleaves C-terminal amino acid residues and is involved in neuropeptide processing, is itself subject to intracellular processing Carboxypeptidase E (CPE) is an enkephalin-convertase that cleaved a C-terminal basic residue from enkephalin precursors to generate enkephalin[1].CPE is a glycoprotein, which is synthesized as a larger inactive precursor. It is exclusively synthesized by membrane-bound polyribosomes in the brain[1][2]. There exists two forms of CPE, soluble and membrane-bound in most tissues with CPE activity. These two forms differ slightly in molecular weight, but have identical enzymatic properties. Both forms arise from the same precursor, which is encoded by a single gene. This gene is a member of a carboxypeptidase gene family that includes CPA and CPB[2].
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P16870-1 (R42-S453)
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Molecular Construction
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N-term
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CPE (R42-S453)
Accession # P16870-1 -
6*His
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C-term
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Protein Length
Partial
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Synonyms
CPE; Insulin Granule-Associated Carboxypeptidase; Carboxypeptidase E; CPH; Enkephalin Convertase; Epididymis Secretory Sperm Binding Protein; Carboxypeptidase H; Carbocypeptidase E; Prohormone-Processing Carboxypeptidase; IDDHH; Cobalt-Stimulated Chromaffin Granule Carboxypeptidase; BDVS
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AA Sequence
RLQQEDGISFEYHRYPELREALVSVWLQCTAISRIYTVGRSFEGRELLVIELSDNPGVHEPGEPEFKYIGNMHGNEAVGRELLIFLAQYLCNEYQKGNETIVNLIHSTRIHIMPSLNPDGFEKAASQPGELKDWFVGRSNAQGIDLNRNFPDLDRIVYVNEKEGGPNNHLLKNMKKIVDQNTKLAPETKAVIHWIMDIPFVLSANLHGGDLVANYPYDETRSGSAHEYSSSPDDAIFQSLARAYSSFNPAMSDPNRPPCRKNDDDSSFVDGTTNGGAWYSVPGGMQDFNYLSSNCFEITVELSCEKFPPEETLKTYWEDNKNSLISYLEQIHRGVKGFVRDLQGNPIANATISVEGIDHDVTSAKDGDYWRLLIPGNYKLTASAPGYLAITKKVAVPYSPAAGVDFELESFS
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Predicted Molecular Mass
47.2 kDa
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Molecular Weight
Approximately 50-60 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
1.Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, 1 mM ZnCl2, 10% glycerol, pH 7.5.
2.Supplied as a 0.22 μm filtered solution of 20 mM PB, 500 mM NaCl, 0.05% Tween 80, 250 mM Glycine, 2 mM EDTA, 50 mM L-Arg, pH 6.5.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. E Manser, et al. Human carboxypeptidase E. Isolation and characterization of the cDNA, sequence conservation, expression and processing in vitroBiochem J. 1990 Apr 15;267(2):517-25. [Content Brief]
[2]. Lloyd D Fricker, et al.Carboxypeptidase E and the Identification of Novel Neuropeptides as Potential Therapeutic Targets. Adv Pharmacol [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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