Carboxypeptidase M/CPM Protein, Mouse (HEK293, His)
Carboxypeptidase M/CPM Protein, Mouse (HEK293, His) is an approximately 56.0 kDa mouse carboxypeptidase M protein with a His-flag. Carboxypeptidase M/CPM is a membrane-bound arginine/lysine carboxypeptidase enzyme belongs to the carboxypeptidases family.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Carboxypeptidase M/CPM Protein, Mouse (HEK293, His) is an approximately 56.0 kDa mouse carboxypeptidase M protein with a His-flag. Carboxypeptidase M/CPM is a membrane-bound arginine/lysine carboxypeptidase enzyme belongs to the carboxypeptidases family[1].
Background
Carboxypeptidase M is a membrane-bound arginine/lysine carboxypeptidase enzyme, cleaves C-terminal basic amino acids with a neutral pH optimum[1]. Carboxypeptidase M belongs to the carboxypeptidases family. Carboxypeptidase M is widely distributed in a variety of tissues and cells, and it constitutively expresses in an active form at the surface of specialized cells and tissues in the human body[2]. Carboxypeptidase M plays an important role in the physiological processes of blood coagulation/fibrinolysis, inflammation, food digestion and pro-hormone and neuropeptide processing[3].
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-6*His
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Accession
Q80V42-1 (L18-S423)
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Molecular Construction
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N-term
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Cpm (L18-S423)
Accession # Q80V42-1 -
6*His
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C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
CPM; Urinary Carboxypeptidase B; Carboxypeptidase M; Renal Carboxypeptidase
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AA Sequence
LDFRYHHQEGMEAFLKSVAQNYSSITHLHSIGKSVRGRNLWVLVVGQTPKEHRVGIPEFKYVANMHGDETVGRELLLHLIDYLVSSYRKDPEITHLIDSTRIHIMPSMNPDGFEAVQKPDCYYSNGRENYNNYDLNRNFPDAFENNNVTKQPETLAIMEWLKTETFVLSANLHGGALVASYPFDNGVQATGTLLSRSLTPDDDVFQHLAYTYASRNPNMTKGDQCKNKRNFPNGIINGYSWYPLQGGMQDYNYIWAQCFEITLELSCCKYPREEKLPLFWNDNKASLIEYIKQVHLGVKGQVFDQSGAPLPNVIVEVQDRKHICPFRTNKLGEYYLLLLPGSYVINVTVPGHDSYLTKLTIPGKSQPFSALKKDFHLPLRWQPDSISVSNPSCPMIPLYKFMPSHS
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Predicted Molecular Mass
47.5 kDa
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Molecular Weight
Approximately 52-56 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
References
[1]. A Nagae, et al. Carboxypeptidase M in brain and peripheral nerves. J Neurochem [Content Brief]
[2]. Kathleen Deiteren, et al. Carboxypeptidase M: Multiple alliances and unknown partners. Clin Chim Acta. 2009 Jan;399(1-2):24-39. [Content Brief]
[3]. David Reverter, Crystal structure of human carboxypeptidase M, a membrane-bound enzyme that regulates peptide hormone activity. J Mol Biol [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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