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Cathepsin C/DPPI Protein, Human (HEK293, C-His)

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Based on 1 Customer Validation

Cathepsin C/DPPI Protein, a thiol protease, exhibits dipeptidylpeptidase activity with broad substrate specificity, excluding proline at the P1 position and arginine at the P2 position. Operating as both exopeptidase and endopeptidase, it displays catalytic versatility. Crucially, Cathepsin C/DPPI activates serine proteases like elastase, cathepsin G, and granzymes A and B. Cathepsin C/DPPI Protein, Human (HEK293, C-His) is the recombinant human-derived Cathepsin C/DPPI protein, expressed by HEK293, with C-10*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Cathepsin C/DPPI Protein, Human (HEK293, C-His)
  • Cathepsin C/DPPI Protein, Human (HEK293, C-His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Cathepsin C/DPPI Protein, a thiol protease, exhibits dipeptidylpeptidase activity with broad substrate specificity, excluding proline at the P1 position and arginine at the P2 position. Operating as both exopeptidase and endopeptidase, it displays catalytic versatility. Crucially, Cathepsin C/DPPI activates serine proteases like elastase, cathepsin G, and granzymes A and B. Cathepsin C/DPPI Protein, Human (HEK293, C-His) is the recombinant human-derived Cathepsin C/DPPI protein, expressed by HEK293, with C-10*His labeled tag.

Background

Cathepsin C/DPPI Protein functions as a thiol protease, displaying dipeptidylpeptidase activity with a broad substrate specificity encompassing both polar and hydrophobic amino acids. Notably, proline is restricted from the P1 position, and arginine cannot occupy the P2 position of the substrate. This enzyme operates as both an exopeptidase and an endopeptidase, showcasing versatility in its catalytic functions. Of significance, Cathepsin C/DPPI plays a crucial role in the activation of serine proteases, including elastase, cathepsin G, and granzymes A and B.

Verified Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate 10 μM Gly-Arg-AMC (HY-P4342) that incubate at room temperature in kinetic Mode for 5 minutes. The specific activity is > 200 pmol/min/µg. (Activation description: The proenzyme needs to be activated by Human Cathepsin L1 (HY-P7754A) for an activated form.)

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    P53634-1/NP_001805.3 (D25-L463)

  • Gene ID
    1075
  • Molecular Construction
    • N-term
    • Cathepsin C/DPPI (D25-L463)
      Accession # P53634-1/NP_001805.3
    • 10*His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    CTSC; Dipeptidyl Peptidase I; Prev. PALS; CPPI; Prev. PLS; DPPI; Dipeptidyl Peptidase 1; Dipeptidyl Peptidase 1 Isoform A Preproprotein; Dipeptidyl Transferase; PDON1; Cathepsin J; HMS; DPP1; JPD; Dipeptidyl-Peptidase I; JP; Cathepsin C; DPP-I

  • AA Sequence

    DTPANCTYLDLLGTWVFQVGSSGSQRDVNCSVMGPQEKKVVVYLQKLDTAYDDLGNSGHFTIIYNQGFEIVLNDYKWFAFFKYKEEGSKVTTYCNETMTGWVHDVLGRNWACFTGKKVGTASENVYVNIAHLKNSQEKYSNRLYKYDHNFVKAINAIQKSWTATTYMEYETLTLGDMIRRSGGHSRKIPRPKPAPLTAEIQQKILHLPTSWDWRNVHGINFVSPVRNQASCGSCYSFASMGMLEARIRILTNNSQTPILSPQEVVSCSQYAQGCEGGFPYLIAGKYAQDFGLVEEACFPYTGTDSPCKMKEDCFRYYSSEYHYVGGFYGGCNEALMKLELVHHGPMAVAFEVYDDFLHYKKGIYHHTGLRDPFNPFELTNHAVLLVGYGTDSASGMDYWIVKNSWGTGWGENGYFRIRRGTDECAIESIAVAATPIPKL

  • Predicted Molecular Mass

    51 kDa

  • Molecular Weight

    Approximately 55-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution in 20 mM MES, 150 mM NaCl, pH 5.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Cathepsin C/DPPI Protein, Human (HEK293, C-His) Product Image
    1/16
  • Experimental Validation Results for Cathepsin C/DPPI Protein, Human (HEK293, C-His)
  • Cathepsin C/DPPI Protein, Human (HEK293, C-His) Product Image