CCL9/10/MIP-1 gamma Protein, Mouse

CCL9 Protein, Mouse, is often produced in macrophages, osteoclasts, and functions as a cell survival factor.

CCL9, a chemokine also known as macrophage inflammatory protein-1 gamma (MIP-1γ), is often produced in macrophages, osteoclasts, and functions as a cell survival factor. Among CC chemokine family members, the mouse CCL9 shares 45, 24, and 20% aa sequence identity with mouse CCL6 (C10), CCL3 (MIP-1α), and CCL4 (MIP-1β), respectively. CCL9/CCR1 signaling is important for the recruitment of myeloid progenitors to intestinal tumors, leading to enhanced invasion. CCL9 is highly induced in immature myeloid cells in the premetastatic lung of tumor-bearing mice or in co-culture with tumor cells, and it plays a critical role in tumor cell survival at the metastatic site^[1][2].

1.The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/mL.
2.Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is ≤0.34 μg/mL, corresponding to a specific activity is ≥2.94×10³ U/mg.

• 
  Measured by its ability to chemoattract THP‑1 human acute monocytic leukemia cells. The ED50 for this effect is 0.34 μg/mL, corresponding to a specific activity is 2.94×103 U/mg.

Mouse

E. coli

Tag Free

P51670 (Q22-Q122)

20308  [NCBI]

QITHATETKEVQSSLKAQQGLEIEMFHMGFQDSSDCCLSYNSRIQCSRFIGYFPTSGGCTRPGIIFISKRGFQVCANPSDRRVQRCIERLEQNSQPRTYKQ

Approximately 12-15 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized after extensive dialysis against 20 mM Tris-HCl, 300 mM NaCl, pH 8.0 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Hangyi H Yan, et al. CCL9 Induced by TGFβ Signaling in Myeloid Cells Enhances Tumor Cell Survival in the Premetastatic Organ. Cancer Res. 2015 Dec 15;75(24):5283-98.  [Content Brief]

  [2]. Xinyan Zhao, et al. CCL9 is secreted by the follicle-associated epithelium and recruits dome region Peyer's patch CD11b+ dendritic cells. J Immunol. 2003 Sep 15;171(6):2797-803.  [Content Brief]

  [3]. Baohua Li, et al. CCL9/CCR1 induces myeloid?derived suppressor cell recruitment to the spleen in a murine H22 orthotopic hepatoma model. Oncol Rep. 2019 Jan;41(1):608-618.  [Content Brief]