MME Protein, Cynomolgus (HEK293, His)

MME Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived MME protein, expressed by HEK293, with N-His tag.

The MME Protein demonstrates a thermolysin-like specificity, predominantly acting on polypeptides of up to 30 amino acids. Biologically crucial, it plays a key role in the degradation of opioid peptides, including Met- and Leu-enkephalins, achieved through the cleavage of a Gly-Phe bond. Additionally, MME catalyzes the cleavage of bradykinin, substance P, and neurotensin peptides and is capable of cleaving angiotensin-1, angiotensin-2, and angiotensin 1-9. The protein is involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). Furthermore, MME displays UV-inducible elastase activity toward skin preelastic and elastic fibers.

Cynomolgus

HEK293

N-8*His

G7NZK8 (D53-W750)

101925772

Partial

DDGICKSSDCIKSAARLIQNMDATAEPCADFFKYACGGWLKRNVIPETSSRYGNFDILRDELEVVLKDVLQEPKTEDIVAVQKAKTLYRSCINESAIDSRGGEPLLKLLPDIYGWPVATENWEQKYGASWTAEKAIAQLNSKYGKKVLINLFVGTDDKNSVNHVIHIDQPRLGLPSRDYYECTGIYKEACTAYVDFMISVARLIRQEERLPIDENQLALEMNKVMELEKEIANATAKPEDRNDPMLLYNKMTLAQLQSNFSLEINGKPFSWLNFTNEIMSTVNISITNEEDVVVYAPEYLTKLKPILTKYSARDLQNLMSWRFIMDLVSSLSRTYKESRNAFRKALYGTTSETATWRRCANYVNGNMENAVGRLYVEAAFAGESKHVVEDLIAQIREVFIQTLDDLTWMDAETKKRAEEKALAIKERIGYPDDIVSNDNKLNNEYLELNYKEDEYFENIIQNLKFSQSKQLKKLREKVDKDEWISGAAVVNAFYSSGRNQIVFPAGILQPPFFSAQQSNSLNYGGIGMVIGHEITHGFDDNGRNFNKDGDLVDWWTQQSASNFKEQSQCMVYQYGNFSWDLAGGQHLNGINTLGENIADNGGIGQAYRAYQNYVKKNGEEKLLPGLDLNHKQLFFLNFAQVWCGTYRPEYAVNSIKTDVHSPGNFRIIGTLQNSAEFSEAFHCRKNSYMNPEKKCRVW

Approximately 81-110 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

≥ 95%, as determined by Bis-Tris PAGE.

<1 EU/μg, determined by LAL method.