CD19 Protein, Human (HEK293, Fc)

The CD19 protein is a B cell antigen receptor coreceptor that enhances downstream signaling pathways, lowers the activation threshold, and helps B cells respond to antigens. It activates phosphatidylinositol 3-kinase and mobilizes intracellular Ca(2+). CD19 Protein, Human (HEK293, Fc) is the recombinant human-derived CD19 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD19 Protein, Human (HEK293, Fc) is 272 a.a., with molecular weight of ~85.0 kDa.

The CD19 Protein serves as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes, playing a pivotal role in decreasing the threshold for activation of downstream signaling pathways and facilitating B-cell responses to antigens. It activates signaling pathways leading to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca(2+) stores. Although not required for early steps during B cell differentiation in the blood marrow, CD19 is essential for the normal differentiation of B-1 cells. Moreover, it is crucial for normal B cell differentiation and proliferation in response to antigen challenges, influencing serum immunoglobulin levels and the production of high-affinity antibodies in response to antigen challenge. CD19 forms complexes with CR2/CD21, CD81, and IFITM1/CD225 in the membrane of mature B-cells. It interacts directly with CD81, a crucial interaction for trafficking and compartmentalization of the CD19 receptor on the cell surface of activated B cells. Additionally, CD19 interacts with VAV, GRB2, SOS, PLCG2, LYN, and the regulatory p85 subunit of phosphatidylinositol 3-kinase when phosphorylated on specific tyrosine residues.

Human

HEK293

C-hFc

P15391 (P20-K291)

930  [NCBI]

PEEPLVVKVEEGDNAVLQCLKGTSDGPTQQLTWSRESPLKPFLKLSLGLPGLGIHMRPLAIWLFIFNVSQQMGGFYLCQPGPPSEKAWQPGWTVNVEGSGELFRWNVSDLGGLGCGLKNRSSEGPSSPSGKLMSPKLYVWAKDRPEIWEGEPPCLPPRDSLNQSLSQDLTMAPGSTLWLSCGVPPDSVSRGPLSWTHVHPKGPKSLLSLELKDDRPARDMWVMETGLLLPRATAQDAGKYYCHRGNLTMSFHLEITARPVLWHWLLRTGGWK

Approximately 85.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.