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  2. CD Antigens
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  5. CD1A-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His)

CD1A-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His)

Cat. No.: HY-P76208
Handling Instructions Technical Support

CD1A-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived CD1A-B2M Heterodimer protein, expressed by HEK293, with C-His labeled tag., has molecular weight of ~45.2 (33.6+11.6) KDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

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Description

CD1A-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived CD1A-B2M Heterodimer protein, expressed by HEK293, with C-His labeled tag., has molecular weight of ~45.2 (33.6+11.6) KDa.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized Rhesus Macaque CD1A-B2M Heterodimer, at 2μg/mL (100μL/well) can bind Anti-CD1A antibody. The ED50 for this effect is 0.1894 μg/mL.

  • Measured by its binding ability in a functional ELISA. Immobilized Rhesus Macaque CD1A-B2M Heterodimer, at 2 μg/mL (100μL/well) can bind Anti-CD1A antibody. The ED50 for this effect is 0.1894 μg/mL.
Species

Rhesus Macaque

Source

HEK293

Tag

C-6*His

Accession

NP_001139290 (D19-S299)&NP_001040602 (I21-M119)

Gene ID
Synonyms
T-cell surface glycoprotein CD1a; CD1A; Beta-2-microglobulin; B2M
Molecular Weight

Approximately 33-50 & 12 kDa due to the glycosylation.

Glycosylation
Yes
Purity

≥ 95%, as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CD1A-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD1A-B2M Heterodimer Protein, Rhesus Macaque (HEK293, His)
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HY-P76208
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