1. Recombinant Proteins
  2. Immune Checkpoint Proteins CAR-T related Proteins CD Antigens Biotinylated Proteins
  3. Inhibitory Checkpoint Molecules Macrophage CD Proteins Monocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins
  4. B7-H3 B7-H3/CD276
  5. CD276/B7-H3 Protein, Human (Biotinylated, HEK293, His-Avi)

CD276/B7-H3 Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P78074
COA Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
20 μg $245 In-stock
50 μg $540 In-stock
100 μg $918 Get quote
250 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Biological Activity

1. Immobilized Biotinylated Human B7-H3 (4Ig) His at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-B7-H3 Ab hFc with the EC50 ≤ 15.2 ng/mL determined by ELISA.
2. Immobilized Anti-B7-H3 Antibody, hFc Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Biotinylated Human B7-H3 (4Ig) , His Tag with the EC50 ≤ 9.7 ng/mL determined by ELISA.








Q5ZPR3 (G27-T461)

Gene ID
B7-H3; CD276; PSEC0249;  4Ig-B7-H3; UNQ309; PRO352; B7 homolog 3; CD276
Molecular Weight

70-80 kDa


Greater than 95% as determined by Tris-Bis PAGE.


Lyophilized powder.


Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.


It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.


Room temperature in continental US; may vary elsewhere.

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name



Applicant Name *


Email Address *

Phone Number *


Organization Name *

Department *


Requested quantity *

Country or Region *



Bulk Inquiry

Inquiry Information

Product Name:
CD276/B7-H3 Protein, Human (Biotinylated, HEK293, His-Avi)
Cat. No.:
MCE Japan Authorized Agent: